Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Once and only once: mechanisms of centriole duplication and their deregulation in disease

EA Nigg, AJ Holland - Nature reviews Molecular cell biology, 2018 - nature.com
Centrioles are conserved microtubule-based organelles that form the core of the centrosome
and act as templates for the formation of cilia and flagella. Centrioles have important roles in …

The centrosome is a selective condensate that nucleates microtubules by concentrating tubulin

JB Woodruff, BF Gomes, PO Widlund, J Mahamid… - Cell, 2017 - cell.com
Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays.
They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic …

Centrosome function and assembly in animal cells

PT Conduit, A Wainman, JW Raff - Nature reviews Molecular cell …, 2015 - nature.com
It has become clear that the role of centrosomes extends well beyond that of important
microtubule organizers. There is increasing evidence that they also function as coordination …

Mechanism and regulation of centriole and cilium biogenesis

DK Breslow, AJ Holland - Annual review of biochemistry, 2019 - annualreviews.org
The centriole is an ancient microtubule-based organelle with a conserved nine-fold
symmetry. Centrioles form the core of centrosomes, which organize the interphase …

The centrosome and its duplication cycle

J Fu, IM Hagan, DM Glover - Cold Spring Harbor …, 2015 - cshperspectives.cshlp.org
The centrosome was discovered in the late 19th century when mitosis was first described.
Long recognized as a key organelle of the spindle pole, its core component, the centriole …

Microtubule nucleation by γ-tubulin complexes and beyond

CA Tovey, PT Conduit - Essays in biochemistry, 2018 - portlandpress.com
In this short review, we give an overview of microtubule nucleation within cells. It is nearly 30
years since the discovery of γ-tubulin, a member of the tubulin superfamily essential for …

Regulated assembly of a supramolecular centrosome scaffold in vitro

JB Woodruff, O Wueseke, V Viscardi, J Mahamid… - Science, 2015 - science.org
The centrosome organizes microtubule arrays within animal cells and comprises two
centrioles surrounded by an amorphous protein mass called the pericentriolar material …

Structural basis for mitotic centrosome assembly in flies

Z Feng, A Caballe, A Wainman, S Johnson… - Cell, 2017 - cell.com
Summary In flies, Centrosomin (Cnn) forms a phosphorylation-dependent scaffold that
recruits proteins to the mitotic centrosome, but how Cnn assembles into a scaffold is unclear …

A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion

Y Zheng, RA Buchwalter, C Zheng, EM Wight… - Nature cell …, 2020 - nature.com
Non-centrosomal microtubule-organizing centres (ncMTOCs) have a variety of roles that are
presumed to serve the diverse functions of the range of cell types in which they are found …