Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Fast-transmitting vertebrate axons are electrically insulated with multiple layers of
nonconductive plasma membrane of glial cell origin, termed myelin. The myelin membrane …

Biological membranes form an essential barrier between living cells and their external
environments, as well as serve to compartmentalize intracellular organelles within …

This review is the continuation of three previously published articles [Jorrin JV, Maldonado
AM, Castillejo MA. Plant proteome analysis: a 2006 update. Proteomics 2007; 7: 2947 …

The need for highly resolving separation methods that can be combined with MS has driven
the development of multidimensional LC. Although the concept is not new, Motoyama and …

Biological research has focused in the past on model organisms and most of the functional
genomics studies in the field of plant sciences are still performed on model species or …

In this century we have assisted at an unimaginable expansion of proteomics, with
continuous innovations and optimizations in methods, techniques, protocols, equipment …

1.1. Biomembranes Biological membranes consist mainly of lipids and proteins (Figure 1)
and form the boundaries of all cells. The major lipids present in biomembranes are …

With the acceleration of proteome research, increasing attention has been paid to
multidimensional liquid chromatography–mass spectrometry (MDLC–MS) due to its high …

The evolutionary conserved protein Cdc48/VCP is involved in various cellular processes,
such as protein degradation, membrane fusion and chaperone activity. Increased levels of …