[HTML][HTML] mRNA-based therapeutics—developing a new class of drugs
In vitro transcribed (IVT) mRNA has recently come into focus as a potential new drug class to
deliver genetic information. Such synthetic mRNA can be engineered to transiently express …
deliver genetic information. Such synthetic mRNA can be engineered to transiently express …
Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems
S Guan, J Rosenecker - Gene therapy, 2017 - nature.com
Because of its safe and effective protein expression profile, in vitro transcribed messenger
RNA (IVT-mRNA) represents a promising candidate in the development of novel …
RNA (IVT-mRNA) represents a promising candidate in the development of novel …
Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
L Mátés, MKL Chuah, E Belay, B Jerchow, N Manoj… - Nature …, 2009 - nature.com
Abstract The Sleeping Beauty (SB) transposon is a promising technology platform for gene
transfer in vertebrates; however, its efficiency of gene insertion can be a bottleneck in …
transfer in vertebrates; however, its efficiency of gene insertion can be a bottleneck in …
Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome Engineering
N Sandoval-Villegas, W Nurieva, M Amberger… - International journal of …, 2021 - mdpi.com
Transposons are mobile genetic elements evolved to execute highly efficient integration of
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …
Sarcoma derived from cultured mesenchymal stem cells
J Tolar, AJ Nauta, MJ Osborn… - Stem …, 2007 - academic.oup.com
To study the biodistribution of MSCs, we labeled adult murine C57BL/6 MSCs with firefly
luciferase and DsRed2 fluorescent protein using nonviral Sleeping Beauty transposons and …
luciferase and DsRed2 fluorescent protein using nonviral Sleeping Beauty transposons and …
Transposon-mediated genome manipulation in vertebrates
Transposable elements are DNA segments with the unique ability to move about in the
genome. This inherent feature can be exploited to harness these elements as gene vectors …
genome. This inherent feature can be exploited to harness these elements as gene vectors …
Gene transfer into genomes of human cells by the sleeping beauty transposon system
Abstract The Sleeping Beauty (SB) transposon system, derived from teleost fish sequences,
is extremely effective at delivering DNA to vertebrate genomes, including those of humans …
is extremely effective at delivering DNA to vertebrate genomes, including those of humans …
Harnessing a high cargo-capacity transposon for genetic applications in vertebrates
Viruses and transposons are efficient tools for permanently delivering foreign DNA into
vertebrate genomes but exhibit diminished activity when cargo exceeds 8 kilobases (kb) …
vertebrate genomes but exhibit diminished activity when cargo exceeds 8 kilobases (kb) …
High-resolution genome-wide mapping of transposon integration in mammals
SR Yant, X Wu, Y Huang, B Garrison… - … and cellular biology, 2005 - Am Soc Microbiol
Abstract The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene
discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions …
discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions …
Sleeping beauty transposition: biology and applications for molecular therapy
Transposable elements can be considered as natural, nonviral gene-delivery vehicles and
are valuable and widely used tools for germ-line transgenesis and insertional mutagenesis …
are valuable and widely used tools for germ-line transgenesis and insertional mutagenesis …