A comprehensive overview of cotton genomics, biotechnology and molecular biological studies

X Wen, Z Chen, Z Yang, M Wang, S Jin, G Wang… - Science China Life …, 2023 - Springer
Cotton is an irreplaceable economic crop currently domesticated in the human world for its
extremely elongated fiber cells specialized in seed epidermis, which makes it of high …

[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants

MM Hassan, Y Zhang, G Yuan, K De, JG Chen… - Trends in Plant …, 2021 - cell.com
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Miniature type VF CRISPR-Cas nucleases enable targeted DNA modification in cells

G Bigelyte, JK Young, T Karvelis, K Budre… - Nature …, 2021 - nature.com
Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools

J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …

Genome editing for plant research and crop improvement

X Zhan, Y Lu, JK Zhu, JR Botella - Journal of Integrative Plant …, 2021 - Wiley Online Library
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

GhWRKY41 forms a positive feedback regulation loop and increases cotton defence response against Verticillium dahliae by regulating phenylpropanoid metabolism

S Xiao, Y Ming, Q Hu, Z Ye, H Si, S Liu… - Plant biotechnology …, 2023 - Wiley Online Library
Despite the established significance of WRKY proteins and phenylpropanoid metabolism in
plant immunity, how WRKY proteins modulate aspects of the phenylpropanoid pathway …

CRISPR/Cas genome editing technologies for plant improvement against biotic and abiotic stresses: advances, limitations, and future perspectives

Y Wang, N Zafar, Q Ali, H Manghwar, G Wang, L Yu… - Cells, 2022 - mdpi.com
Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to
improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game …

Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit …

T Xin, H Tian, Y Ma, S Wang, L Yang, X Li… - Horticulture …, 2022 - academic.oup.com
Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon,
and squash, contribute greatly to the human diet. The widespread use of genome editing …

Expanding the plant genome editing toolbox with recently developed CRISPR–Cas systems

N Wada, K Osakabe, Y Osakabe - Plant Physiology, 2022 - academic.oup.com
Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

Molecular evolution and functional modification of plant miRNAs with CRISPR

F Deng, F Zeng, Q Shen, A Abbas, J Cheng… - Trends in plant …, 2022 - cell.com
Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-
associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic …