Molecular mechanisms underlying microtubule growth dynamics

JM Cleary, WO Hancock - Current Biology, 2021 - cell.com
Microtubules are dynamic cytoskeletal filaments composed of αβ-tubulin heterodimers.
Historically, the dynamics of single tubulin interactions at the growing microtubule tip have …

Measuring microtubule dynamics

AJ Zwetsloot, G Tut, A Straube - Essays in biochemistry, 2018 - portlandpress.com
Microtubules are key players in cellular self-organization, acting as structural scaffolds,
cellular highways, force generators and signalling platforms. Microtubules are polar …

The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability

J Roostalu, C Thomas, NI Cade, S Kunzelmann… - Elife, 2020 - elifesciences.org
Microtubules are cytoskeletal polymers whose function depends on their property to switch
between states of growth and shrinkage. Growing microtubules are thought to be stabilized …

The size of the EB cap determines instantaneous microtubule stability

C Duellberg, NI Cade, D Holmes, T Surrey - Elife, 2016 - elifesciences.org
The function of microtubules relies on their ability to switch between phases of growth and
shrinkage. A nucleotide-dependent stabilising cap at microtubule ends is thought to be lost …

Microtubule aging probed by microfluidics-assisted tubulin washout

C Duellberg, NI Cade, T Surrey - Molecular biology of the cell, 2016 - Am Soc Cell Biol
Microtubules switch stochastically between phases of growth and shrinkage. The molecular
mechanism responsible for the end of a growth phase, an event called catastrophe, is still …

[HTML][HTML] Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: A review

B Özdemir, R Reski - Computational and structural biotechnology journal, 2021 - Elsevier
Cytoskeletal filaments are structures of utmost importance to biological cells and organisms
due to their versatility and the significant functions they perform. These biopolymers are most …

MTrack: automated detection, tracking, and analysis of dynamic microtubules

V Kapoor, WG Hirst, C Hentschel, S Preibisch… - Scientific reports, 2019 - nature.com
Microtubules are polar, dynamic filaments fundamental to many cellular processes. In vitro
reconstitution approaches with purified tubulin are essential to elucidate different aspects of …

Measurements and simulations of microtubule growth imply strong longitudinal interactions and reveal a role for GDP on the elongating end

JM Cleary, T Kim, WO Hancock, LM Rice - Biophysical Journal, 2022 - cell.com
2517-Pos The effect of ionic strength in microtubule tactoid formation Prashali Chauhan,
Hong Beom Lee, Niaz Goodbee, Ruell Branch, Sumon Sahu, Jennifer Schwarz, Jennifer L …

Steady-state EB cap size fluctuations are determined by stochastic microtubule growth and maturation

J Rickman, C Duellberg, NI Cade… - Proceedings of the …, 2017 - National Acad Sciences
Growing microtubules are protected from depolymerization by the presence of a GTP or
GDP/Pi cap. End-binding proteins of the EB1 family bind to the stabilizing cap, allowing …

Interactions of tropomyosin Tpm1. 1 on a single actin filament: A method for extraction and processing of high resolution TIRF microscopy data

M Janco, T Böcking, S He, ACF Coster - PLoS One, 2018 - journals.plos.org
Skeletal muscle tropomyosin (Tpm1. 1) is an elongated, rod-shaped, alpha-helical coiled-
coil protein that forms continuous head-to-tail polymers along both sides of the actin …