Small-angle X-ray and neutron scattering

CM Jeffries, J Ilavsky, A Martel, S Hinrichs… - Nature Reviews …, 2021 - nature.com
Small-angle scattering (SAS) is a technique that is able to probe the structural organization
of matter and quantify its response to changes in external conditions. X-ray and neutron …

Visualizing transient dark states by NMR spectroscopy

NJ Anthis, GM Clore - Quarterly Reviews of Biophysics, 2015 - cambridge.org
Myriad biological processes proceed through states that defy characterization by
conventional atomic-resolution structural biological methods. The invisibility of these …

Sequence‐specific determination of protein and peptide concentrations by absorbance at 205 nm

NJ Anthis, GM Clore - Protein Science, 2013 - Wiley Online Library
Quantitative studies in molecular and structural biology generally require accurate and
precise determination of protein concentrations, preferably via a method that is both quick …

Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca2+-ligated calmodulin

T Schmidt, J Jeon, WM Yau… - Proceedings of the …, 2022 - National Acad Sciences
Recent advances in rapid mixing and freeze quenching have opened the path for time-
resolved electron paramagnetic resonance (EPR)-based double electron-electron …

Super-resolution in solution X-ray scattering and its applications to structural systems biology

RP Rambo, JA Tainer - Annual review of biophysics, 2013 - annualreviews.org
Small-angle X-ray scattering (SAXS) is a robust technique for the comprehensive structural
characterizations of biological macromolecular complexes in solution. Here, we present a …

Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS)

MA Graewert, DI Svergun - Current opinion in structural biology, 2013 - Elsevier
Highlights•Popularity of SAXS/WAXS for structural analysis has gained substantially.•
Remarkable developments in hardware and software allow for high-throughput …

Biological small-angle neutron scattering: recent results and development

E Mahieu, F Gabel - Acta Crystallographica Section D: Structural …, 2018 - journals.iucr.org
Small-angle neutron scattering (SANS) has increasingly been used by the structural biology
community in recent years to obtain low-resolution information on solubilized …

Millisecond time-resolved solid-state NMR reveals a two-stage molecular mechanism for formation of complexes between calmodulin and a target peptide from …

J Jeon, WM Yau, R Tycko - Journal of the American Chemical …, 2020 - ACS Publications
Calmodulin (CaM) mediates a wide range of biological responses to changes in intracellular
Ca2+ concentrations through its calcium-dependent binding affinities to numerous target …

Co-micellization conduct and structural dynamics of block copolymers in water and salt solution environment for drug solubilization enhancement

D Patel, SL Gawali, K Kuperkar, PA Hassan… - Colloid and Polymer …, 2023 - Springer
The present study examines the co-micellization in different block copolymers (BCPs): P123
and F127 (with almost similar hydrophobic PO block but different hydrophilic% EO content) …

Interdomain dynamics explored by paramagnetic NMR

L Russo, M Maestre-Martinez, S Wolff… - Journal of the …, 2013 - ACS Publications
An ensemble-based approach is presented to explore the conformational space sampled by
a multidomain protein showing moderate interdomain dynamics in terms of translational and …