Data obtained while investigating growth plate chondrocyte differentiation during
endochondral bone formation both in vivo and in vitro indicate that initial chondrogenesis …

Long-term primary culture of mammalian cells has been always difficult due to unavoidable
senescence. Conventional methods for generating immortalized cell lines usually require …

Immortalized human chondrocytes were established by transfection of primary cultures of
juvenile costal chondrocytes with vectors encoding simian virus 40 large T antigen and …

Cell number is often a limiting factor in studies of chondrocyte physiology, particularly for
human investigations. Chondrocytes can be readily proliferated in monolayer culture …

The differentiated phenotype of chondrocyte is rapidly lost during in vitro culture by a
process designated" dedifferentiation." In this study, we investigate the roles of protein …

Cells isolated from the trabecular meshwork (TM) of a male glaucoma patient were
transformed by transfection with an origin defective mutant of S V40 virus. Transformation …

Chondrocytes cultivated in monolayer rapidly divide and lose their morphological and
biochemical characteristics, whereas they maintain their phenotype for long periods of time …

Objective To develop a reproducible immortalized human chondrocyte culture model for
studying the regulation of chondrocyte functions relevant to arthritic diseases in adult …

An infinite cell line is one of the most favored experimental tools and plays an irreplaceable
role in cell-based biological research. Primary cells from normal animal tissues undergo a …

Primary cell culture in vitro suffers from cellular senescence. We hypothesized that
expansion on decellularized extracellular matrix (dECM) deposited by simian virus 40 large …