Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
Recent advances in improving gene-editing specificity through CRISPR–Cas9 nuclease engineering
CRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in
many areas. For safe therapeutic applications in clinical medicine, its off-target effect must …
many areas. For safe therapeutic applications in clinical medicine, its off-target effect must …
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics
The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins.
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …
CRISPR nuclease off-target activity and mitigation strategies
B Wienert, MK Cromer - Frontiers in Genome Editing, 2022 - frontiersin.org
The discovery of CRISPR has allowed site-specific genomic modification to become a reality
and this technology is now being applied in a number of human clinical trials. While this …
and this technology is now being applied in a number of human clinical trials. While this …
Systems analysis of highly multiplexed CRISPR-base editing in streptomycetes
CRISPR tools, especially Cas9n-sgRNA guided cytidine deaminase base editors such as
CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One …
CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One …
Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces
J Wang, K Wang, Z Deng, Z Zhong, G Sun… - Nature …, 2024 - nature.com
Base editing (BE) faces protospacer adjacent motif (PAM) constraints and off-target effects in
both eukaryotes and prokaryotes. For Streptomyces, renowned as one of the most prolific …
both eukaryotes and prokaryotes. For Streptomyces, renowned as one of the most prolific …
Recent advances in genome-editing technology with CRISPR/Cas9 variants and stimuli-responsive targeting approaches within tumor cells: a future perspective of …
The innovative advances in transforming clustered regularly interspaced short palindromic
repeats-associated protein 9 (CRISPR/Cas9) into different variants have taken the art of …
repeats-associated protein 9 (CRISPR/Cas9) into different variants have taken the art of …
Comparative analysis of CRISPR off-target discovery tools following ex vivo editing of CD34+ hematopoietic stem and progenitor cells
While a number of methods exist to investigate CRISPR off-target (OT) editing, few have
been compared head-to-head in primary cells after clinically relevant editing processes …
been compared head-to-head in primary cells after clinically relevant editing processes …
CRISPR/Cas9 landscape: Current state and future perspectives
M Tyumentseva, A Tyumentsev, V Akimkin - International Journal of …, 2023 - mdpi.com
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a unique
genome editing tool that can be easily used in a wide range of applications, including …
genome editing tool that can be easily used in a wide range of applications, including …
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets
Abstract Streptococcus pyogenes Cas9 (SpCas9) has been employed as a genome
engineering tool with a promising potential within therapeutics. However, its off-target effects …
engineering tool with a promising potential within therapeutics. However, its off-target effects …