The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Since the advent of induced pluripotent stem cell (iPSC) technology a decade ago,
enormous progress has been made in stem cell biology and regenerative medicine. Human …

Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of
canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based …

The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional
repressor, but inefficiencies in target-gene silencing have limited its utility. Here we describe …

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

CRISPR/Cas9-based functional genomics have transformed our ability to elucidate
mammalian cell biology. However, most previous CRISPR-based screens were conducted …

Human cortical organoids (hCOs), derived from human embryonic stem cells (hESCs),
provide a platform to study human brain development and diseases in complex three …

INTRODUCTION The human genome contains tens of thousands of loci that produce long
noncoding RNAs (lncRNAs), transcripts that have no apparent protein-coding potential. A …

Epidemiological studies reveal that marijuana increases the risk of cardiovascular disease
(CVD); however, little is known about the mechanism. Δ 9-tetrahydrocannabinol (Δ 9-THC) …

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA. Here,
we build on this observation with a comprehensive algorithm that incorporates chromatin …