As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …

Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …

Metabolic engineering of microorganisms to produce desirable products on an industrial
scale can result in unbalanced cellular metabolic networks that reduce productivity and …

Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

Genome engineering of Corynebacterium glutamicum, an important industrial
microorganism for amino acids production, currently relies on random mutagenesis and …

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated
nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established …

The establishment of a clustered regularly interspaced short palindromic repeat (CRISPR)-
Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward …

Rho is a general transcription termination factor playing essential roles in RNA polymerase
(RNAP) recycling, gene regulation, and genomic stability in most bacteria. Traditional …

Background Corynebacterium glutamicum is an important industrial workhorse and
advanced genetic engineering tools are urgently demanded. Recently, the clustered …