New imaging methodologies with high contrast and molecular specificity allow researchers
to analyze dynamic processes in plant cells at multiple scales, from single protein and RNA …

Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Expansion microscopy (ExM) has gained increasing popularity for 3D ultrastructural imaging
of cultured cells and tissue slices at nanoscale resolution using conventional microscopes …

In this work, we have developed an expansion microscopy (ExM) protocol that combines
ExM with photoactivated localization microscopy (ExPALM) for yeast cell imaging, and report …

Expansion microscopy (ExM) is a promising technology that enables nanoscale imaging on
conventional optical microscopes by physically magnifying the specimens. Here, we report …

Recent studies have shown that mitochondrial morphology can modulate organelle function
and greatly affect stem cell behavior, thus affecting tissue homeostasis. As such, we …

CA2 is an understudied subregion of the hippocampus that is critical for social memory.
Previous studies identified multiple components of the mitochondrial calcium uniporter …

Ultrastructure expansion microscopy (U‐ExM) involves the physical magnification of
specimens embedded in hydrogels, which allows for super‐resolution imaging of …

Protein retention expansion microscopy (ExM) retains fluorescent signals in fixed tissue and
isotropically expands the tissue to allow nanoscale (< 70 nm) resolution on diffraction-limited …

One aspect of the invention provides a method for preparing a biological sample. The
method includes: exposing the biological sample to a photocrosslinkable substance …