Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments

ME Kandel, YR He, YJ Lee, THY Chen… - Nature …, 2020 - nature.com
Due to its specificity, fluorescence microscopy has become a quintessential imaging tool in
cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit …

Label-free SARS-CoV-2 detection and classification using phase imaging with computational specificity

N Goswami, YR He, YH Deng, C Oh, N Sobh… - Light: Science & …, 2021 - nature.com
Efforts to mitigate the COVID-19 crisis revealed that fast, accurate, and scalable testing is
crucial for curbing the current impact and that of future pandemics. We propose an optical …

Perspectives in Genome-Editing Techniques for Livestock

J Popova, V Bets, E Kozhevnikova - Animals, 2023 - mdpi.com
Simple Summary Genetically modified farm animals have been actively used in a number of
applications from testing gene functions to increasing production and economic value of …

Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure

ME Kandel, M Rubessa, YR He… - Proceedings of the …, 2020 - National Acad Sciences
The ability to evaluate sperm at the microscopic level, at high-throughput, would be useful
for assisted reproductive technologies (ARTs), as it can allow specific selection of sperm …

Comparison of two methods of sperm‐and testis‐mediated gene transfer in production of transgenic animals: A systematic review

Z Dehghan, G Darya, S Mehdinejadiani… - Animal …, 2024 - Wiley Online Library
Transgenic (Tg) animal technology is one of the growing areas in biology. Various Tg
technologies, each with its own advantages and disadvantages, are available for generating …

Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos

MB Wheeler, RAC Rabel, M Rubessa… - Biology of …, 2024 - academic.oup.com
Assisted reproduction is one of the significant tools to treat human infertility. Morphological
assessment is the primary method to determine sperm and embryo viability during IVF …

[HTML][HTML] The effect of methyl-beta-cyclodextrin on DNA absorption and quality of posttransfected sperm

M Rahimi, S Rahimi, M Sharafi, A Shahverdi, JL Grimes - Poultry Science, 2021 - Elsevier
Sperm can be selected as a natural vector for the production of transgenic animals. Methyl-
beta-cyclodextrin (MBCD) removes cholesterol from the phospholipid membrane of sperm …

Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy

M Rubessa, ME Kandel, S Schreiber… - Systems Biology in …, 2020 - Taylor & Francis
The goal of this study was to characterize sperm populations resulting from three different
methods of sperm selection used for bovine in vitro fertilization. We compared sperm …

Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

M Rubessa, MB Wheeler - Theriogenology, 2020 - Elsevier
In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various
species. However, there is no consensus or gold standard method on how to assess their …

Rapid SARS-CoV-2 Detection and Classification Using Phase Imaging with Computational Specificity

N Goswami, YR He, YH Deng, C Oh, N Sobh, E Valera… - bioRxiv, 2020 - biorxiv.org
Efforts to mitigate the COVID-19 crisis revealed that fast, accurate, and scalable testing is
crucial for curbing the current impact and that of future pandemics. We propose an optical …