Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications

J Sankaran, T Wohland - Communications Biology, 2023 - nature.com
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

Fluorescence, phosphorescence, and chemiluminescence

N Siraj, B El-Zahab, S Hamdan, TE Karam… - Analytical …, 2016 - ACS Publications
Fluorescence, Phosphorescence, and Chemiluminescence cover a wide array of topics.
One can simply examine the number of topics that arise from photon induced excitation and …

An alternative framework for fluorescence correlation spectroscopy

S Jazani, I Sgouralis, OM Shafraz, S Sivasankar… - Biophysical …, 2019 - cell.com
Fluorescence correlation spectroscopy (FCS) is a flexible and widely used tool routinely
exploited in vivo. While FCS provides estimates of dynamical quantities, such as diffusion …

Fluorescence correlation spectroscopy: The technique and its applications in soft matter

A Gupta, J Sankaran, T Wohland - Physical Sciences Reviews, 2019 - degruyter.com
Fluorescence correlation spectroscopy (FCS) is a well-established single-molecule method
used for the quantitative spatiotemporal analysis of dynamic processes in a wide range of …

Plasma membrane organization of epidermal growth factor receptor in resting and ligand-bound states

N Bag, S Huang, T Wohland - Biophysical journal, 2015 - cell.com
The spatial arrangement of the epidermal growth factor receptor (EGFR) on the cellular
plasma membrane is one of the prime factors that control its downstream signaling pathways …

[HTML][HTML] Fluorescence strategies for mapping cell membrane dynamics and structures

J Sankaran, T Wohland - APL bioengineering, 2020 - pubs.aip.org
Fluorescence spectroscopy has been a cornerstone of research in membrane dynamics and
organization. Technological advances in fluorescence spectroscopy went hand in hand with …

The secreted signaling protein Wnt3 is associated with membrane domains in vivo: a SPIM-FCS study

XW Ng, C Teh, V Korzh, T Wohland - Biophysical journal, 2016 - cell.com
Wnt3 is a morphogen that activates the Wnt signaling pathway and regulates a multitude of
biological processes ranging from cell proliferation and cell fate specification to …

Single molecule data analysis: An introduction

M Tavakoli, JN Taylor, CB Li… - … in Chemical Physics, 2017 - Wiley Online Library
This chapter considers statistical data‐driven analysis methods, and focuses on parametric
as well as more recent information theoretic and nonparametric statistical approaches to …

Wnt3 is lipidated at conserved cysteine and serine residues in zebrafish neural tissue

D Dhasmana, S Veerapathiran, Y Azbazdar… - Frontiers in Cell and …, 2021 - frontiersin.org
Wnt proteins are a family of hydrophobic cysteine-rich secreted glycoproteins that regulate a
gamut of physiological processes involved in embryonic development and tissue …

The epidermal growth factor receptor forms location-dependent complexes in resting cells

S Yavas, R Macháň, T Wohland - Biophysical journal, 2016 - cell.com
The epidermal growth factor receptor (EGFR) is a prototypical receptor tyrosine kinase
involved in cell growth and proliferation and associated with various cancers. It is commonly …