Heterologous protein production in filamentous fungi

D Liu, S Garrigues, RP de Vries - Applied microbiology and biotechnology, 2023 - Springer
Filamentous fungi are able to produce a wide range of valuable proteins and enzymes for
many industrial applications. Recent advances in fungal genomics and experimental …

Strategies for gene disruption and expression in filamentous fungi

YZ Mei, YL Zhu, PW Huang, Q Yang, CC Dai - Applied microbiology and …, 2019 - Springer
Filamentous fungi can produce many valuable secondary metabolites; among these fungi,
endophytic fungi play an ecological role in mutualistic symbiosis with plants, including …

FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi

E Moreno-Giménez, M Gandía, Z Sáez… - … in bioengineering and …, 2023 - frontiersin.org
Fungal synthetic biology is a rapidly expanding field that aims to optimize the
biotechnological exploitation of fungi through the generation of standard, ready-to-use …

Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity

S Garrigues, M Gandía, L Castillo, M Coca… - Frontiers in …, 2018 - frontiersin.org
Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich
proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different …

Characterization of a fungal competition factor: production of a conidial cell-wall associated antifungal peptide

S Tong, M Li, NO Keyhani, Y Liu, M Yuan, D Lin… - PLoS …, 2020 - journals.plos.org
Competition is one of the fundamental driving forces of natural selection. Beauveria
bassiana is a soil and plant phylloplane/root fungus capable of parasitizing insect hosts. Soil …

[PDF][PDF] Calixarene-mediated assembly of a small antifungal protein

JM Alex, ML Rennie, S Engilberge, G Lehoczki… - IUCrJ, 2019 - journals.iucr.org
Synthetic macrocycles such as calixarenes and cucurbiturils are increasingly applied as
mediators of protein assembly and crystallization. The macrocycle can facilitate assembly by …

New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176

A Huber, D Hajdu, D Bratschun-Khan, Z Gáspári… - Scientific Reports, 2018 - nature.com
Small, cysteine-rich and cationic proteins with antimicrobial activity are produced by diverse
organisms of all kingdoms and represent promising molecules for drug development. The …

Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay

M Gandía, A Kakar, M Giner-Llorca, J Holzknecht… - Journal of Fungi, 2021 - mdpi.com
Penicillium phytopathogenic species provoke severe postharvest disease and economic
losses. Penicillium expansum is the main pome fruit phytopathogen while Penicillium …

Buckwheat Antifungal Protein with Biocontrol Potential To Inhibit Fungal (Botrytis cinerea) Infection of Cherry Tomato

C Wang, S Yuan, W Zhang, T Ng… - Journal of agricultural and …, 2019 - ACS Publications
A 11 kDa antifungal protein FEAP was purified from buckwheat (Fagopyrum esculentum)
seed extract with a procedure involving (NH4) 2SO4 precipitation and chromatography on …

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus

X Shi, T Cordero, S Garrigues… - Plant biotechnology …, 2019 - Wiley Online Library
Fungi that infect plants, animals or humans pose a serious threat to human health and food
security. Antifungal proteins (AFP s) secreted by filamentous fungi are promising …