Illuminating RNA biology through imaging

P Le, N Ahmed, GW Yeo - Nature cell biology, 2022 - nature.com
RNA processing plays a central role in accurately transmitting genetic information into
functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to …

Recent advances in plasma-engineered polymers for biomarker-based viral detection and highly multiplexed analysis

SM Mousavi, SA Hashemi, MY Kalashgrani, A Gholami… - Biosensors, 2022 - mdpi.com
Infectious diseases remain a pervasive threat to global and public health, especially in many
countries and rural urban areas. The main causes of such severe diseases are the lack of …

Mobile platform for rapid sub–picogram-per-milliliter, multiplexed, digital droplet detection of proteins

V Yelleswarapu, JR Buser, M Haber… - Proceedings of the …, 2019 - National Acad Sciences
Digital droplet assays—in which biological samples are compartmentalized into millions of
femtoliter-volume droplets and interrogated individually—have generated enormous …

Subcellular detection of SARS-CoV-2 RNA in human tissue reveals distinct localization in alveolar type 2 pneumocytes and alveolar macrophages

KK Acheampong, DL Schaff, BL Emert, J Lake… - MBio, 2022 - Am Soc Microbiol
The widespread coronavirus disease 2019 (COVID-19) is caused by infection with the novel
coronavirus SARS-CoV-2. Currently, we have limited understanding of which cells become …

Amplified Fluorescence in Situ Hybridization by Small and Bright Dye-Loaded Polymeric Nanoparticles

S Egloff, N Melnychuk, E Cruz Da Silva, A Reisch… - ACS …, 2021 - ACS Publications
Detection and imaging of RNA at the single-cell level is of utmost importance for
fundamental research and clinical diagnostics. Current techniques of RNA analysis …

Sensitive visualization of SARS-CoV-2 RNA with CoronaFISH

E Rensen, S Pietropaoli, F Mueller… - Life Science …, 2022 - life-science-alliance.org
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2). The positive-sense single-stranded RNA virus contains a …

[HTML][HTML] FISH and chips: a review of microfluidic platforms for FISH analysis

P Rodriguez-Mateos, NF Azevedo, C Almeida… - Medical microbiology …, 2020 - Springer
Fluorescence in situ hybridization (FISH) allows visualization of specific nucleic acid
sequences within an intact cell or a tissue section. It is based on molecular recognition …

A magnetic micropore chip for rapid (< 1 hour) unbiased circulating tumor cell isolation and in situ RNA analysis

J Ko, N Bhagwat, SS Yee, T Black, C Redlinger… - Lab on a Chip, 2017 - pubs.rsc.org
The use of microtechnology for the highly selective isolation and sensitive detection of
circulating tumor cells has shown enormous promise. One challenge for this technology is …

Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2

NR Sundah, A Natalia, Y Liu, NRY Ho, H Zhao… - Science …, 2021 - science.org
Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current
detection approaches remain limited due to their high complexity and extensive processing …

Visualizing adenosine-to-inosine RNA editing in single mammalian cells

IA Mellis, R Gupte, A Raj, SH Rouhanifard - Nature methods, 2017 - nature.com
Conversion of adenosine to inosine is a frequent type of RNA editing, but important details
about the biology of this conversion remain unknown because of a lack of imaging tools. We …