Structural proteomics approaches using mass spectrometry are increasingly used in biology
to examine the composition and structure of macromolecules. Hydroxyl radical–mediated …

Actin, one of the main proteins of muscle and cytoskeleton, exists as a vaiety of highly
conserved isoforms whose distribution in vertebrates is tissue-specific. Synthesis of specific …

A nucleotide-dependent conformational change regulates actin filament dynamics. Yet, the
structural basis of this mechanism remains controversial. The x-ray crystal structure of …

Mutations in the skeletal muscle alpha-actin gene (ACTA1) associated with congenital
myopathy with excess of thin myofilaments, nemaline myopathy and intranuclear rod …

The structure of an “open state” of crystalline profilin: β-actin has been solved to 2.65 Å by X-
ray crystallography. The open-state crystals, in 1.8 M potassium phosphate, have an …

Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we
generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by …

Actin filament growth and disassembly, as well as affinity for actin-binding proteins, is
mediated by the nucleotide-bound state of the component actin monomers. The structural …

The ability of actin to both polymerize into filaments and to depolymerize permits the rapid
rearrangements of actin structures that are essential for actin's function in most cellular …

This minireview is dedicated to the memory of Henryk Eisenberg and honors his major
contributions to many areas of biophysics and to the analysis of macromolecular states and …

In actin from many species H73 is methylated, but the function of this rare post-translational
modification is unknown. Although not within bonding distance, it is located close to the γ …