Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food
industries, in addition to being a daily consideration for academic researchers studying …

Enzymes generated by natural recruitment and protein engineering have greatly contribute
in various sets of applications. However, their insufficient stability is a bottleneck that limit the …

Buffers comprise an integral component of protein formulations. Not only do they function to
regulate shifts in pH, they also can stabilize proteins by a variety of mechanisms. The ability …

The flavoenzyme dihydroorotate dehydrogenase catalyzes the stereoselective oxidation of
(S)-dihydroorotate to orotate in the fourth of the six conserved enzymatic reactions involved …

Differential scanning fluorimetry (DSF), also known as ThermoFluor or Thermal Shift Assay,
has become a commonly-used approach for detecting protein-ligand interactions …

Introduction: Macromolecular X-ray crystallography has been the primary methodology for
determining the three-dimensional structures of proteins, nucleic acids and viruses …

Enzymes expressed by highly salt-tolerant organisms show many modifications compared
with salt-affected counterparts including biased amino acid and lower α-helix content, lower …

All biological processes rely on the formation of protein–ligand, protein–peptide and protein–
protein complexes. Studying the affinity, kinetics and thermodynamics of binding between …

Programmed cell death is a tightly controlled process critical for the removal of damaged or
infected cells. Pro-and antiapoptotic proteins of the Bcl-2 family are pivotal mediators of this …

Cytochrome c oxidase (COX) assembly factor 7 (COA7) is a metazoan-specific assembly
factor, critical for the biogenesis of mitochondrial complex IV (cytochrome c oxidase) …