Current research and future perspectives of phytase bioprocessing

K Bhavsar, JM Khire - RSC advances, 2014 - pubs.rsc.org
Phosphorus is one of nature's paradoxes as it is life's bottleneck for subsistence on earth but
at same time is detrimental in surplus quantities in an aquatic environment. Phosphorus …

Recent advances in directed phytase evolution and rational phytase engineering

AV Shivange, U Schwaneberg - Directed enzyme evolution: advances and …, 2017 - Springer
Phytases are hydrolytic enzymes that initiate stepwise removal of phosphate from phytate.
Phytate is the major phosphorous storage compound in cereal gains, oilseeds, and legumes …

Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis

Z Tang, W Jin, R Sun, Y Liao, T Zhen, H Chen… - Enzyme and microbial …, 2018 - Elsevier
We previously constructed three recombinant phyA mutant strains (PP-NP m-8, PP-NP ep-
6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of …

Improving the activity and expression level of a phthalate-degrading enzyme by a combination of mutagenesis strategies and strong promoter replacement

S Sun, Y Tan, L Wang, Z Wu, J Zhou, G Wu… - … Science and Pollution …, 2023 - Springer
Phthalic acid esters (PAEs) are widely used plasticizers found in consumer products, which
enter the environment and pose severe threats to human health. Here, a new PAE …

[图书][B] Directed enzyme evolution: Advances and applications

M Alcalde - 2017 - Springer
The last couple of decades have witnessed a true revolution in protein engineering that very
few could have foreseen, a revolution delivered by the hand of directed evolution. This …

Enhancement of Thermostability and Kinetic Efficiency of Aspergillus niger PhyA Phytase by Site-Directed Mutagenesis

A Hesampour, SER Siadat, MA Malboobi… - Applied biochemistry …, 2015 - Springer
Phytase efficiently catalyzes the hydrolysis of phytate to phosphate; it can be utilized as an
animal supplement to provide animals their nutrient requirements for phosphate and to …

Integrative structural and computational biology of phytases for the animal feed industry

NG Nezhad, RNZ Raja Abd Rahman, YM Normi… - Catalysts, 2020 - mdpi.com
Resistance to high temperature, acidic pH and proteolytic degradation during the pelleting
process and in the digestive tract are important features of phytases as animal feed. The …

Enhanced activity of an alkaline phytase from Bacillus subtilis 168 in acidic and neutral environments by directed evolution

W Chen, L Ye, F Guo, Y Lv, H Yu - Biochemical Engineering Journal, 2015 - Elsevier
As a feed additive, the desirable features of phytase include thermostability, strict substrate
specificity, and high stability in a wide pH range. Alkaline phytases from Bacillus subtilis are …

Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R

Y Liao, C Li, H Chen, Q Wu, Z Shan, X Han - Applied biochemistry and …, 2013 - Springer
Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the
bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain …

Furfural-tolerant Zymomonas mobilis derived from error-prone PCR-based whole genome shuffling and their tolerant mechanism

S Huang, T Xue, Z Wang, Y Ma, X He, J Hong… - Applied microbiology …, 2018 - Springer
Furfural-tolerant strain is essential for the fermentative production of biofuels or chemicals
from lignocellulosic biomass. In this study, Zymomonas mobilis CP4 was for the first time …