Current research and future perspectives of phytase bioprocessing
K Bhavsar, JM Khire - RSC advances, 2014 - pubs.rsc.org
Phosphorus is one of nature's paradoxes as it is life's bottleneck for subsistence on earth but
at same time is detrimental in surplus quantities in an aquatic environment. Phosphorus …
at same time is detrimental in surplus quantities in an aquatic environment. Phosphorus …
Recent advances in directed phytase evolution and rational phytase engineering
AV Shivange, U Schwaneberg - Directed enzyme evolution: advances and …, 2017 - Springer
Phytases are hydrolytic enzymes that initiate stepwise removal of phosphate from phytate.
Phytate is the major phosphorous storage compound in cereal gains, oilseeds, and legumes …
Phytate is the major phosphorous storage compound in cereal gains, oilseeds, and legumes …
Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis
Z Tang, W Jin, R Sun, Y Liao, T Zhen, H Chen… - Enzyme and microbial …, 2018 - Elsevier
We previously constructed three recombinant phyA mutant strains (PP-NP m-8, PP-NP ep-
6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of …
6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of …
Improving the activity and expression level of a phthalate-degrading enzyme by a combination of mutagenesis strategies and strong promoter replacement
S Sun, Y Tan, L Wang, Z Wu, J Zhou, G Wu… - … Science and Pollution …, 2023 - Springer
Phthalic acid esters (PAEs) are widely used plasticizers found in consumer products, which
enter the environment and pose severe threats to human health. Here, a new PAE …
enter the environment and pose severe threats to human health. Here, a new PAE …
[图书][B] Directed enzyme evolution: Advances and applications
M Alcalde - 2017 - Springer
The last couple of decades have witnessed a true revolution in protein engineering that very
few could have foreseen, a revolution delivered by the hand of directed evolution. This …
few could have foreseen, a revolution delivered by the hand of directed evolution. This …
Enhancement of Thermostability and Kinetic Efficiency of Aspergillus niger PhyA Phytase by Site-Directed Mutagenesis
A Hesampour, SER Siadat, MA Malboobi… - Applied biochemistry …, 2015 - Springer
Phytase efficiently catalyzes the hydrolysis of phytate to phosphate; it can be utilized as an
animal supplement to provide animals their nutrient requirements for phosphate and to …
animal supplement to provide animals their nutrient requirements for phosphate and to …
Integrative structural and computational biology of phytases for the animal feed industry
Resistance to high temperature, acidic pH and proteolytic degradation during the pelleting
process and in the digestive tract are important features of phytases as animal feed. The …
process and in the digestive tract are important features of phytases as animal feed. The …
Enhanced activity of an alkaline phytase from Bacillus subtilis 168 in acidic and neutral environments by directed evolution
W Chen, L Ye, F Guo, Y Lv, H Yu - Biochemical Engineering Journal, 2015 - Elsevier
As a feed additive, the desirable features of phytase include thermostability, strict substrate
specificity, and high stability in a wide pH range. Alkaline phytases from Bacillus subtilis are …
specificity, and high stability in a wide pH range. Alkaline phytases from Bacillus subtilis are …
Site-Directed Mutagenesis Improves the Thermostability and Catalytic Efficiency of Aspergillus niger N25 Phytase Mutated by I44E and T252R
Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the
bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain …
bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain …
Furfural-tolerant Zymomonas mobilis derived from error-prone PCR-based whole genome shuffling and their tolerant mechanism
S Huang, T Xue, Z Wang, Y Ma, X He, J Hong… - Applied microbiology …, 2018 - Springer
Furfural-tolerant strain is essential for the fermentative production of biofuels or chemicals
from lignocellulosic biomass. In this study, Zymomonas mobilis CP4 was for the first time …
from lignocellulosic biomass. In this study, Zymomonas mobilis CP4 was for the first time …