Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read
lengths than the second-generation sequencing (SGS) technologies, making it well-suited …

The rapid development of next-generation sequencing (NGS) technology, including
advances in sequencing chemistry, sequencing technologies, bioinformatics, and data …

One-third of all protein-coding genes from bacterial genomes cannot be annotated with a
function. Here, to investigate the functions of these genes, we present genome-wide mutant …

Abstract Motivation: Single Molecule Real-Time (SMRT) sequencing technology and Oxford
Nanopore technologies (ONT) produce reads over 10 kb in length, which have enabled high …

Almost half the cells and 1% of the unique genes found in our bodies are human, the rest
are from microbes, predominantly bacteria, archaea, fungi, and viruses. These …

Motivation: PacBio single molecule real-time sequencing is a third-generation sequencing
technique producing long reads, with comparatively lower throughput and higher error rate …

We present a hierarchical genome-assembly process (HGAP) for high-quality de novo
microbial genome assemblies using only a single, long-insert shotgun DNA library in …

Lyme disease (Lyme borreliosis) is a tick-borne, zoonosis of adults and children caused by
genospecies of the Borrelia burgdorferi sensu lato complex. The ailment, widespread …

Background DNA sequencing technologies deviate from the ideal uniform distribution of
reads. These biases impair scientific and medical applications. Accordingly, we have …

Petunia hybrida is a popular bedding plant that has a long history as a genetic model
system. We report the whole-genome sequencing and assembly of inbred derivatives of its …