Deaminases, ubiquitous enzymes found in all living organisms from bacteria to humans,
serve diverse and crucial functions. Notably, purine and pyrimidine deaminases, while …

Population-scale genome modification can alter the composition or fate of wild populations.
Synthetic gene drives provide one set of tools, but their use is complicated by scientific …

Cytosine base editing is a powerful tool for making precise single nucleotide changes in
cells and model organisms like zebrafish, which are valuable for studying human diseases …

RNA binding proteins (RBPs) perform a myriad of functions and are implicated in numerous
neurological diseases. To identify the targets of RBPs in small numbers of cells, we …

CRISPR base editors can introduce point mutations into DNA precisely, and cytosine base
editors (CBEs) catalyze C to T transitions. While CBEs have been thoroughly explored in cell …

Target-AID, BE3, and ABE7. 10 base editors fused to the catalytically modified Cas9 and
xCas9 (3.7) were tested for germline editing of the fruit fly Drosophila melanogaster. We …

Understanding how the number, placement and affinity of transcription factor binding sites
dictates gene regulatory programs remains a major unsolved challenge in biology …

Population-scale genome editing can be used to alter the composition or fate of wild
populations. One approach to achieving these aims utilizes a synthetic gene drive element …

This thesis focused on addressing two crucial aspects of circadian biology:(1) temperature
compensation property and,(2) molecular evolution of clock genes/proteins. Additionally, for …

Functional Analysis of Circadian Clock genes in Drosophila melanogaster School of
Doctoral Studies in Biological Sciences University of South Bohemia in České Budějovice …