Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Fluorescence in nanobiotechnology: sophisticated fluorophores for novel applications

B Hötzer, IL Medintz, N Hildebrandt - Small, 2012 - Wiley Online Library
Nanobiotechnology is one of the fastest growing and broadest‐ranged interdisciplinary
subfields of the nanosciences. Countless hybrid bio‐inorganic composites are currently …

Versatile protein tagging in cells with split fluorescent protein

D Kamiyama, S Sekine, B Barsi-Rhyne, J Hu… - Nature …, 2016 - nature.com
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has
now seen more and more application of epitope tags. The small size of these tags may …

SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition

D Cieri, M Vicario, M Giacomello, F Vallese… - Cell Death & …, 2018 - nature.com
Contact sites are discrete areas of organelle proximity that coordinate essential
physiological processes across membranes, including Ca2+ signaling, lipid biosynthesis …

[HTML][HTML] Specialized filopodia direct long-range transport of SHH during vertebrate tissue patterning

TA Sanders, E Llagostera, M Barna - Nature, 2013 - nature.com
The ability of signalling proteins to traverse tissues containing tightly packed cells is of
fundamental importance for cell specification and tissue development; however, how this is …

[HTML][HTML] A split horseradish peroxidase for the detection of intercellular protein–protein interactions and sensitive visualization of synapses

JD Martell, M Yamagata, TJ Deerinck, S Phan… - Nature …, 2016 - nature.com
Intercellular protein–protein interactions (PPIs) enable communication between cells in
diverse biological processes, including cell proliferation, immune responses, infection, and …

Characterization and mechanism of stress-induced translocation of 78-kilodalton glucose-regulated protein (GRP78) to the cell surface

YL Tsai, Y Zhang, CC Tseng, R Stanciauskas… - Journal of Biological …, 2015 - ASBMB
Glucose-regulated protein (GRP78)/BiP, a major chaperone in the endoplasmic reticulum, is
recently discovered to be preferably expressed on the surface of stressed cancer cells …

Split green fluorescent proteins: scope, limitations, and outlook

MG Romei, SG Boxer - Annual review of biophysics, 2019 - annualreviews.org
Many proteins can be split into fragments that spontaneously reassemble, without covalent
linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment …

Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds

T Köker, N Tang, C Tian, W Zhang, X Wang… - Nature …, 2018 - nature.com
The in cellulo assembly of plasmonic nanomaterials into photo-responsive probes is of great
interest for many bioimaging and nanophotonic applications but remains challenging with …

Single-molecule fluorescence imaging in living cells

T Xia, N Li, X Fang - Annual review of physical chemistry, 2013 - annualreviews.org
The transition of single-molecule fluorescence detection and imaging from in vitro to living
cells has greatly enriched our knowledge on the behavior of single biomolecules in their …