Directed evolution has proved to be an effective strategy for improving or altering the activity
of biomolecules for industrial, research and therapeutic applications. The evolution of …

Molecular biologists and chemists alike have long sought to modify proteins with
substituents that cannot be installed by standard or even advanced genetic approaches. We …

Interactions between different cell types are essential for multiple biological processes,
including immunity, embryonic development and neuronal signalling. Although the …

Highlights•Sortase-catalyzed ligation is a powerful strategy for protein modification.•
Engineered sortases improve reaction rates and eliminate Ca 2+ dependency.•Deactivation …

Current therapies for autoimmune diseases rely on traditional immunosuppressive
medications that expose patients to an increased risk of opportunistic infections and other …

Bioconjugates represent an emerging class of medicines, which offer therapeutic
opportunities overtaking those of the individual components. Many novel bioconjugates …

Highlights•Enzymatic ligation offers a powerful toolbox for site-selective protein
labelling.•Enzymatic ligation facilitates access to complex protein architectures.•Enzymatic …

Cell–cell interactions and communications play fundamental roles in life processes but
remain largely uncharacterized. We developed an e nzyme-mediated pro x imity cel ll …

Methods to parse paracrine epithelial-stromal communication networks are a vital need in
drug development, as disruption of these networks underlies diseases ranging from cancer …

The renaissance of peptides as prospective therapeutics has fostered the development of
novel strategies for their synthesis and modification. In this context, besides the development …