A comprehensive review of signal peptides: Structure, roles, and applications

H Owji, N Nezafat, M Negahdaripour… - European journal of cell …, 2018 - Elsevier
Signal peptides (SP) are short peptides located in the N-terminal of proteins, carrying
information for protein secretion. They are ubiquitous to all prokaryotes and eukaryotes. SPs …

Signal peptides for recombinant protein secretion in bacterial expression systems

R Freudl - Microbial cell factories, 2018 - Springer
The secretion of biotechnologically or pharmaceutically relevant recombinant proteins into
the culture supernatant of a bacterial expression host greatly facilitates their downstream …

Enhanced Extracellular Production of IsPETase in Escherichia coli via Engineering of the pelB Signal Peptide

L Shi, H Liu, S Gao, Y Weng, L Zhu - Journal of agricultural and …, 2021 - ACS Publications
Poly (ethylene terephthalate)(PET) is one of the most commonly used plastics worldwide
and its accumulation in the environment is a global problem. PETase from Ideonella …

Cutinase: characteristics, preparation, and application

S Chen, L Su, J Chen, J Wu - Biotechnology advances, 2013 - Elsevier
Abstract Cutinases (EC 3.1. 1.74) belong to the α/β-hydrolase superfamily. They were
initially discovered because they are secreted by fungi to hydrolyze the ester bonds of the …

Bacillus subtilis: from soil bacterium to super-secreting cell factory

JM van Dijl, M Hecker - Microbial cell factories, 2013 - Springer
The biotechnology industry has become a key element in modern societies. Within this
industry, the production of recombinant enzymes and biopharmaceutical proteins is of major …

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

R Biedendieck, T Knuuti, SJ Moore, D Jahn - Applied microbiology and …, 2021 - Springer
Over 30 years, the Gram-positive bacterium Priestia megaterium (previously known as
Bacillus megaterium) was systematically developed for biotechnological applications …

Signal peptides generated by attention-based neural networks

Z Wu, KK Yang, MJ Liszka, A Lee, A Batzilla… - ACS Synthetic …, 2020 - ACS Publications
Short (15–30 residue) chains of amino acids at the amino termini of expressed proteins
known as signal peptides (SPs) specify secretion in living cells. We trained an attention …

Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

W Cui, L Han, F Suo, Z Liu, L Zhou, Z Zhou - World Journal of Microbiology …, 2018 - Springer
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

Tat-Independent Secretion of Polyethylene Terephthalate Hydrolase PETase in Bacillus subtilis 168 Mediated by Its Native Signal Peptide

X Huang, L Cao, Z Qin, S Li, W Kong… - Journal of agricultural …, 2018 - ACS Publications
Widespread utilization of polyethylene terephthalate (PET) has caused critical
environmental pollution. The enzymatic degradation of PET is a promising solution to this …

Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides

C Degering, T Eggert, M Puls, J Bongaerts… - Applied and …, 2010 - Am Soc Microbiol
Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial
production of proteins. These strains can efficiently secrete proteins into the culture medium …