Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Oleaginous microbes represent an attractive means of converting a diverse range of
feedstocks into oils that can be transesterified to biodiesel. However, the mechanism of lipid …

Highlights•13 C tracer analysis can be used to probe intracellular metabolism in
cells.•Steady state labeling patterns can quantify nutrient contributions.•Steady state labeling …

Polyamides are important industrial polymers. Currently, they are produced exclusively from
petrochemical monomers. Herein, we report the production of a novel bio-nylon, PA5. 10 …

Stable isotope, and in particular 13C-based flux analysis, is the exclusive approach to
experimentally quantify the integrated responses of metabolic networks. Here we describe a …

Many properties of complex networks cannot be understood from monitoring the
components—not even when comprehensively monitoring all protein or metabolite …

Metabolic engineering is the directed improvement of cellular properties through the
modification of specific biochemical reactions or the introduction of new ones, with the use of …

In many organisms, metabolite interconversion at the phosphoenolpyruvate (PEP)–pyruvate–
oxaloacetate node involves a structurally entangled set of reactions that interconnects the …

Comprehensive knowledge regarding Saccharomyces cerevisiae has accumulated over
time, and today S. cerevisiae serves as a widley used biotechnological production organism …