Biocatalysts are creating increased interest among researchers due to their unique
properties. Several enzymes are efficiently produced by microorganisms. However, the use …

Abstract Adeno-associated virus (AAV)-based vectors are promising tools for gene
therapeutic applications, in part because AAVs are nonpathogenic viruses, and vectors …

Phytases are phosphohydrolytic enzymes that initiate stepwise removal of phosphate from
phytate. Simple-stomached species such as swine, poultry, and fish require extrinsic …

Designing protein sequences with desired biological function is crucial in biology and
chemistry. Recent machine learning methods use a surrogate sequence-function model to …

Enzymes are widely applied in various industrial applications and processes, including the
food and beverage, animal feed, textile, detergent and medical industries. Enzymes …

Successful evolutionary enzyme engineering requires a high throughput screening or
selection method, which considerably increases the chance of obtaining desired properties …

Plastics are highly durable and widely used materials. Current methodologies of plastic
degradation, elimination, and recycling are flawed. In recent years, biodegradation (the …

Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive
alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen …

Additive or non‐additive, that is the question: The increasing awareness of non‐additive
cooperative mutational effects in protein engineering is currently providing new theoretical …

The field of directed evolution has progressed to the point where it is feasible to engineer
enzymes for unnatural substrates and reactions with catalytic efficiencies and regio …