Enteropathogenic Escherichia coli and enterohemorrhagic E. coli are diarrheagenic
bacterial human pathogens that cause severe gastroenteritis. These enteric pathotypes …

Since their discovery, nanobodies have been used extensively in the fields of research,
diagnostics and therapy. These antigen binding fragments, originating from Camelid heavy …

Drug platforms that enable the directed delivery of therapeutics to sites of diseases to
maximize efficacy and limit off-target effects are needed. Here, we report the development of …

Libraries of well-characterised components regulating gene expression levels are essential
to many synthetic biology applications. While widely available for the Gram-negative model …

In vivo mutagenesis systems accelerate directed protein evolution but often show restricted
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …

It was already clinically apparent 150 years ago that bacterial therapy could alleviate
diseases. Recently, a burgeoning number of researchers have been using bacterial …

Infections with many Gram-negative pathogens, including Escherichia coli, Salmonella,
Shigella, and Yersinia, rely on type III secretion system (T3SS) effectors. We hypothesized …

Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

The intestinal epithelial cells (IECs) that line the gut form a robust line of defense against
ingested pathogens. We investigated the impact of infection with the enteric pathogen …

Enteropathogenic E. coli (EPEC) is a human pathogen that causes acute and chronic
pediatric diarrhea. The hallmark of EPEC infection is the formation of attaching and effacing …