Biology spans a continuum of length and time scales. Individual experimental methods only
glimpse discrete pieces of this spectrum but can be combined to construct a more holistic …

The combination of cryo-electron tomography and subtomogram analysis affords 3D high-
resolution views of biological macromolecules in their native cellular environment, or in situ …

Previously we showed that 2D template matching (2DTM) can be used to localize
macromolecular complexes in images recorded by cryogenic electron microscopy (cryo-EM) …

Protein synthesis is central to life and requires the ribosome, which catalyzes the stepwise
addition of amino acids to a polypeptide chain by undergoing a sequence of structural …

Cryogenic electron microscopy (cryo-EM) is a key method in structural and cell biology.
Analysis of cryo-EM images requires interpretation of noisy, low-resolution densities, which …

Determining the structure and mechanisms of all individual functional modules of cells at
high molecular detail has often been seen as equal to understanding how cells work …

Highlights•In situ cryo-ET provides novel mechanistic insights into neuronal biology.•Cryo-
ET enables cellular structural studies of neurodegeneration.•Recent developments allow …

Changing environmental conditions necessitate rapid adaptation of cytoplasmic and nuclear
volumes. We use the slime mold Dictyostelium discoideum, known for its ability to tolerate …

Structural biology is a field of research that investigates the structure and organization of
macromolecules to decipher their functional mechanisms. Traditionally, it has adopted a …

MolecularWebXR is our new website for education, science communication and scientific
peer discussion in chemistry and biology built on WebXR. It democratizes multi-user …