Data‐independent acquisition‐based SWATH‐MS for quantitative proteomics: a tutorial

C Ludwig, L Gillet, G Rosenberger, S Amon… - Molecular systems …, 2018 - embopress.org
Many research questions in fields such as personalized medicine, drug screens or systems
biology depend on obtaining consistent and quantitatively accurate proteomics data from …

Quantitative mass spectrometry-based proteomics: an overview

S Rozanova, K Barkovits, M Nikolov, C Schmidt… - Quantitative methods in …, 2021 - Springer
In recent decades, mass spectrometry has moved more than ever before into the front line of
protein-centered research. After being established at the qualitative level, the more …

[HTML][HTML] The translational landscape of the human heart

S van Heesch, F Witte, V Schneider-Lunitz, JF Schulz… - Cell, 2019 - cell.com
Gene expression in human tissue has primarily been studied on the transcriptional level,
largely neglecting translational regulation. Here, we analyze the translatomes of 80 human …

Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Targeted data extraction of the MS/MS spectra generated by data-independent acquisition: a new concept for consistent and accurate proteome analysis

LC Gillet, P Navarro, S Tate, H Röst, N Selevsek… - Molecular & Cellular …, 2012 - ASBMB
Most proteomic studies use liquid chromatography coupled to tandem mass spectrometry to
identify and quantify the peptides generated by the proteolysis of a biological sample …

Parallel reaction monitoring for high resolution and high mass accuracy quantitative, targeted proteomics

AC Peterson, JD Russell, DJ Bailey… - Molecular & cellular …, 2012 - ASBMB
Selected reaction monitoring on a triple quadrupole mass spectrometer is currently
experiencing a renaissance within the proteomics community for its, as yet, unparalleled …

Selected reaction monitoring–based proteomics: workflows, potential, pitfalls and future directions

P Picotti, R Aebersold - Nature methods, 2012 - nature.com
Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is
emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is …

A mass spectrometric-derived cell surface protein atlas

D Bausch-Fluck, A Hofmann, T Bock, AP Frei… - PloS one, 2015 - journals.plos.org
Cell surface proteins are major targets of biomedical research due to their utility as cellular
markers and their extracellular accessibility for pharmacological intervention. However …

A repository of assays to quantify 10,000 human proteins by SWATH-MS

G Rosenberger, CC Koh, T Guo, HL Röst, P Kouvonen… - Scientific data, 2014 - nature.com
Mass spectrometry is the method of choice for deep and reliable exploration of the (human)
proteome. Targeted mass spectrometry reliably detects and quantifies pre-determined sets …

Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach

SA Carr, SE Abbatiello, BL Ackermann… - Molecular & Cellular …, 2014 - ASBMB
Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction
monitoring (MRM) to study biological and biomedical questions is well underway in the …