Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

W Cui, L Han, F Suo, Z Liu, L Zhou, Z Zhou - World Journal of Microbiology …, 2018 - Springer
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

Production of recombinant proteins in Bacillus subtilis

W Schumann - Advances in applied microbiology, 2007 - Elsevier
Publisher Summary The classical way of protein purification starting from a large number of
cells producing the protein using its authentic expression signals has been replaced by …

Oral vaccines: A better future of immunization

KWY Kwong, Y Xin, NCY Lai, JCC Sung, KC Wu… - Vaccines, 2023 - mdpi.com
Oral vaccines are gaining more attention due to their ease of administration, lower
invasiveness, generally greater safety, and lower cost than injectable vaccines. This review …

[PDF][PDF] Improving protein production on the level of regulation of both expression and secretion pathways in Bacillus subtilis

Y Song, JM Nikoloff, D Zhan - Journal of microbiology and …, 2015 - koreascience.kr
The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial
candidate for protein expression owing to its single membrane and high capacity of …

Bacillus subtilis spores: A novel microparticle adjuvant which can instruct a balanced Th1 and Th2 immune response to specific antigen

AGC Barnes, V Cerovic, PS Hobson… - European journal of …, 2007 - Wiley Online Library
There is a current need for safe, cheap, and effective vaccine adjuvants, to combine with sub‐
unit antigens to enhance their immunogenicity. In this study we have used probiotic Bacillus …

Gut adhesive Bacillus subtilis spores as a platform for mucosal delivery of antigens

M Tavares Batista, RD Souza, JD Paccez… - Infection and …, 2014 - Am Soc Microbiol
Bacillus subtilis spores have been used as safe and heat-resistant antigen delivery vectors.
Nonetheless, the oral administration of spores typically induces weak immune responses to …

Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria

B Thompson, A Siegel - US Patent 9,845,342, 2017 - Google Patents
2023-01-23 Assigned to GENISYS CREDIT UNION reassignment GENISYS CREDIT UNION
SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ELEMENTAL …

Comparative genomics analysis and transposon mutagenesis provides new insights into high menaquinone-7 biosynthetic potential of Bacillus subtilis natto

C Jiang, G Zhao, H Wang, W Zheng, R Zhang, L Wang… - Gene, 2024 - Elsevier
Abstract This research combined Whole-Genome sequencing, intraspecific comparative
genomics and transposon mutagenesis to investigate the menaquinone-7 (MK-7) synthesis …

Comparison of a Regulated Delayed Antigen Synthesis System with In Vivo-Inducible Promoters for Antigen Delivery by Live Attenuated Salmonella Vaccines

S Wang, Y Li, H Shi, W Sun, KL Roland… - Infection and …, 2011 - Am Soc Microbiol
Induction of strong immune responses against a vectored antigen in hosts immunized with
live attenuated Salmonella vaccines is related in part to the amount of antigen delivered and …

Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles

JD Paccez, HD Nguyen, WB Luiz, RCC Ferreira… - Vaccine, 2007 - Elsevier
Recombinant Bacillus subtilis strains, either in the form of spores or vegetative cells, may be
employed as safe and low-cost vaccine vehicles. In this study, we studied the role of …