Recent Advances in Molecular Genetic Tools for Babesia

H Hakimi, M Asada, S Kawazu - Veterinary Sciences, 2021 - mdpi.com
Development of in vitro culture and completion of genome sequencing of several Babesia
parasites promoted the efforts to establish transfection systems for these parasites to dissect …

To kill a piroplasm: genetic technologies to advance drug discovery and target identification in Babesia

CD Keroack, B Elsworth, MT Duraisingh - International journal for …, 2019 - Elsevier
Babesia parasites infect a diverse range of vertebrate hosts, from penguins to pigs.
Recently, the emergence of zoonotic Babesia infection has been increasing, and the list of …

[HTML][HTML] Overexpression of pigment epithelium-derived factor in placenta-derived mesenchymal stem cells promotes mitochondrial biogenesis in retinal cells

JY Kim, S Park, SH Park, D Lee, GH Kim, JE Noh… - Laboratory …, 2021 - Elsevier
Pigment epithelium-derived factor (PEDF) plays a role in protecting retinal pigment epithelial
(RPE) cells from oxidative stress (OS), a causative factor of RPE cell death. Genetically …

Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni

M Liu, PF Adjou Moumouni, M Asada, H Hakimi… - Parasites & vectors, 2018 - Springer
Background Genetic manipulation techniques, such as transfection, have been previously
reported in many protozoan parasites. In Babesia, stable transfection systems have only …

Establishment of a stable transfection method in Babesia microti and identification of a novel bidirectional promoter of Babesia microti

DK Jaijyan, K Govindasamy, J Singh, S Bhattacharya… - Scientific reports, 2020 - nature.com
Babesia microti, an emerging human pathogen, is primarily transmitted through a bite of an
infected tick and blood transfusions in human. Stable transfection technique has been …

Stable transfection system for Babesia sp. Xinjiang

J Wang, X Wang, G Guan, J Yang, J Liu, A Liu, Y Li… - Parasites & …, 2021 - Springer
Background Stable transfection systems have been described in many protozoan parasites,
including Plasmodium falciparum, Cryptosporidium parvum, Babesia bovis, Babesia ovata …

Transient Transfection of the Zoonotic Parasite Babesia microti

M Liu, S Ji, MA Rizk, PF Adjou Moumouni, EM Galon… - Pathogens, 2020 - mdpi.com
The development of genetic manipulation techniques has been reported in many protozoan
parasites over the past few years. However, these techniques have not been established for …

Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase

B Tuvshintulga, AB Nugraha, T Mizutani, M Liu… - Scientific reports, 2021 - nature.com
Theileria equi, an intraerythrocytic protozoan parasite, causes equine piroplasmosis, a
disease which negatively impacts the global horse industry. Genetic manipulation is one of …

Transient transfection of Babesia ovis using heterologous promoters

C Rosa, M Asada, H Hakimi, A Domingos… - Ticks and tick-borne …, 2019 - Elsevier
Babesia species, etiological agents of babesiosis, a recognized emerging tick-borne
disease, are a significant animal and human health concern with a worldwide socio …

Establishment of a transient transfection system for Babesia sp. Xinjiang using homologous promoters

X Wang, J Wang, J Liu, J Yang, Z Lv, A Liu, Y Li… - Parasitology …, 2021 - Springer
Babesia species, the agentic pathogens of human and animal babesiosis, are spread
worldwide. Over the last decade, genetic manipulation approaches have been applied with …