Next-generation sequencing has revolutionized clinical diagnostic testing. Yet, for a
substantial proportion of patients, sequence information restricted to exons and exon–intron …

In many eukaryotes, including mammals, plants, yeast, and insects, introns can increase
gene expression without functioning as a binding site for transcription factors. This …

Mammalian genomes are pervasively transcribed, to produce thousands of long non-coding
RNAs (lncRNAs),. A few of these lncRNAs have been shown to recruit regulatory complexes …

Noncoding RNAs (ncRNAs) have numerous roles in development and disease, and one of
the prominent roles is to regulate gene expression. A vast number of circular RNAs …

Despite the conventional distinction between them, promoters and enhancers share many
features in mammals, including divergent transcription and similar modes of transcription …

Intermolecular RNA-RNA interactions are used by many noncoding RNAs (ncRNAs) to
achieve their diverse functions. To identify these contacts, we developed a method based on …

The expansion of introns within mammalian genomes poses a challenge for the production
of full-length messenger RNAs (mRNAs), with increasing evidence that these long AT-rich …

The smallest viable unit of life is the cell. From bacteria to mammals, all cells use the same
nucleic acid-based universal code for the maintenance and inheritance of genetic …

Precursor mRNA splicing is one of the most highly regulated processes in metazoan
species. In addition to generating vast repertoires of RNAs and proteins, splicing has a …

The C-terminal domain (CTD) of the RNA polymerase II largest subunit consists of multiple
heptad repeats (consensus Tyr1–Ser2–Pro3–Thr4–Ser5–Pro6–Ser7), varying in number …