Fluorescent proteins and genetically encoded biosensors

M Wang, Y Da, Y Tian - Chemical Society Reviews, 2023 - pubs.rsc.org
The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

Fluorescent proteins and their applications in imaging living cells and tissues

DM Chudakov, MV Matz, S Lukyanov… - Physiological …, 2010 - journals.physiology.org
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Versatile protein tagging in cells with split fluorescent protein

D Kamiyama, S Sekine, B Barsi-Rhyne, J Hu… - Nature …, 2016 - nature.com
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has
now seen more and more application of epitope tags. The small size of these tags may …

A cerato‐platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum

G Yang, L Tang, Y Gong, J Xie, Y Fu, D Jiang… - New …, 2018 - Wiley Online Library
Cerato‐platanin proteins (CP s), which are secreted by filamentous fungi, are phytotoxic to
host plants, but their functions have not been well defined to date. Here we characterized a …

R2R3‐MYB transcription factor MYB6 promotes anthocyanin and proanthocyanidin biosynthesis but inhibits secondary cell wall formation in Populus tomentosa

L Wang, W Lu, L Ran, L Dou, S Yao, J Hu… - The Plant …, 2019 - Wiley Online Library
The secondary cell wall is an important carbon sink in higher plants and its biosynthesis
requires coordination of metabolic fluxes in the phenylpropanoid pathway. In Arabidopsis …

Bimolecular fluorescence complementation (BiFC) analysis: advances and recent applications for genome-wide interaction studies

KE Miller, Y Kim, WK Huh, HO Park - Journal of molecular biology, 2015 - Elsevier
Complex protein networks are involved in nearly all cellular processes. To uncover these
vast networks of protein interactions, various high-throughput screening technologies have …

Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives

Y Kodama, CD Hu - Biotechniques, 2012 - Taylor & Francis
Over the past decade, bimolecular fluorescence complementation (BiFC) has emerged as a
key technique to visualize protein-protein interactions in a variety of model organisms. The …

Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System

B Stynen, H Tournu, J Tavernier… - … and molecular biology …, 2012 - Am Soc Microbiol
The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

The transcription factor MYB115 contributes to the regulation of proanthocyanidin biosynthesis and enhances fungal resistance in poplar

L Wang, L Ran, Y Hou, Q Tian, C Li, R Liu… - New …, 2017 - Wiley Online Library
Proanthocyanidins (PA s) are major defense phenolic compounds in the leaves of poplar
(Populus spp.) in response to abiotic and biotic stresses. Transcriptional regulation of PA …

Split green fluorescent proteins: scope, limitations, and outlook

MG Romei, SG Boxer - Annual review of biophysics, 2019 - annualreviews.org
Many proteins can be split into fragments that spontaneously reassemble, without covalent
linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment …