Protein expression is a critical process in diverse biological systems. For Escherichia coli, a
widely employed microbial host in industrial catalysis and healthcare, researchers often face …

Degeneracy in the genetic code, which enables a single protein to be encoded by a
multitude of synonymous gene sequences, has an important role in regulating protein …

Motivation Poor protein solubility hinders the production of many therapeutic and industrially
useful proteins. Experimental efforts to increase solubility are plagued by low success rates …

Aggregation can be thought of as a form of protein folding in which intermolecular
associations lead to the formation of large, insoluble assemblies. Various types of …

Natural enzymes are delicate biomolecules possessing only marginal thermodynamic
stability. Poorly stable, misfolded, and aggregated proteins lead to huge economic losses in …

Protein solubility is an important thermodynamic parameter that is critical for the
characterization of a protein's function, and a key determinant for the production yield of a …

Motivation Solubility and expression levels of proteins can be a limiting factor for large-scale
studies and industrial production. By determining the solubility and expression directly from …

Understanding protein solubility is essential for their functional applications. Computational
methods for predicting protein solubility are crucial for reducing experimental costs and …

Crystallography may be the gold standard of protein structure determination, but obtaining
the necessary high-quality crystals is also in some ways akin to prospecting for the precious …

The expression of proteins in Escherichia coli is often essential for their characterization,
modification, and subsequent application. Gene sequence is the major factor contributing …