Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy
G Silva, L Poirot, R Galetto, J Smith… - Current gene …, 2011 - ingentaconnect.com
The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …
Functional markers in plants
JR Andersen, T Lübberstedt - Trends in plant science, 2003 - cell.com
Different approaches (including association studies) have recently been adopted for the
functional characterization of allelic variation in plants and to identify sequence motifs …
functional characterization of allelic variation in plants and to identify sequence motifs …
Precise genome modification in the crop species Zea mays using zinc-finger nucleases
Agricultural biotechnology is limited by the inefficiencies of conventional random
mutagenesis and transgenesis. Because targeted genome modification in plants has been …
mutagenesis and transgenesis. Because targeted genome modification in plants has been …
The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution
H Puchta - Journal of experimental botany, 2005 - academic.oup.com
The efficient repair of double-strand breaks (DSBs) in genomic DNA is important for the
survival of all organisms. In recent years, basic mechanisms of DSB repair in somatic plant …
survival of all organisms. In recent years, basic mechanisms of DSB repair in somatic plant …
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
A Lloyd, CL Plaisier, D Carroll… - Proceedings of the …, 2005 - National Acad Sciences
Targeted mutagenesis is an essential tool of reverse genetics that could be used
experimentally to investigate basic plant biology or modify crop plants for improvement of …
experimentally to investigate basic plant biology or modify crop plants for improvement of …
High‐frequency homologous recombination in plants mediated by zinc‐finger nucleases
DA Wright, JA Townsend, RJ Winfrey Jr… - The Plant …, 2005 - Wiley Online Library
Homologous recombination offers great promise for plant genome engineering. This
promise has not been realized, however, because when DNA enters plant cells homologous …
promise has not been realized, however, because when DNA enters plant cells homologous …
Agrobacterium T-DNA integration: molecules and models
T Tzfira, J Li, B Lacroix, V Citovsky - TRENDS in Genetics, 2004 - cell.com
Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule
(T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host …
(T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host …
GENETICALLY ENGINEERED ORGANISMS AND THE ENVIRONMENT: CURRENT STATUS AND RECOMMENDATIONS1
The Ecological Society of America has evaluated the ecological effects of current and
potential uses of field‐released genetically engineered organisms (GEOs), as described in …
potential uses of field‐released genetically engineered organisms (GEOs), as described in …
[PDF][PDF] Gene targeting in plants: 25 years later
Only five years after the initiation of transgenic research in plants, gene targeting (GT) was
achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via …
achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via …
Efficient gene targeting by homologous recombination in rice
R Terada, H Urawa, Y Inagaki, K Tsugane… - Nature biotechnology, 2002 - nature.com
Modification of genes through homologous recombination, termed gene targeting, is the
most direct method to characterize gene function. In higher plants, however, the method is …
most direct method to characterize gene function. In higher plants, however, the method is …