The principles of isothermal titration calorimetry (ITC) and differential scanning calorimetry
(DSC) are reviewed together with the basic thermodynamic formalism on which the two …

The understanding of molecular recognition processes of small ligands and biological
macromolecules requires a complete characterization of the binding energetics and …

Metal ions are required for the growth of all life forms. 1-4 Currently, about half of all proteins
contain metal ions, 5 and most ribozymes cannot function without metal ion (s). 1-3, 6-11 …

Isothermal titration calorimetry (ITC) is a well established technique for the study of
biological interactions. The strength of ITC is that it directly measures enthalpy changes …

Isothermal titration calorimetry (ITC) has become the gold-standard technique for studying
binding processes due to its high precision and sensitivity, as well as its capability for the …

Abiogenic lead (Pb2+), present in the environment in elevated levels due to human
activities, has detrimental effects on human health. Metal-binding sites in proteins have been …

Calcium-and integrin-binding protein (CIB) is a novel member of the helix− loop− helix
family of regulatory calcium-binding proteins which likely has a specific function in …

Examination of the NMR 15N chemical shifts of a number of EF-hand proteins shows that the
shift value for the amido nitrogen of the residue in position 8 of a canonical EF-hand loop (or …

EhCaBP1, a calcium‐binding protein of the parasite Entamoeba histolytica, is known to
participate in cellular processes involving actin filaments. This may be due to its direct …

Trivalent lanthanide cations are extensively being used in biochemical experiments to probe
various dication-binding sites in proteins; however, the factors governing the binding …