Cracking the code: Reprogramming the genetic script in prokaryotes and eukaryotes to harness the power of noncanonical amino acids
C Jann, S Giofré, R Bhattacharjee, EA Lemke - Chemical Reviews, 2024 - ACS Publications
Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …
decades. Incorporating these noncanonical amino acids into proteins enables many …
Upgrading aminoacyl-tRNA synthetases for genetic code expansion
Synthesis of proteins with non-canonical amino acids via genetic code expansion is at the
forefront of synthetic biology. Progress in this field has enabled site-specific incorporation of …
forefront of synthetic biology. Progress in this field has enabled site-specific incorporation of …
Engineering the genetic code in cells and animals: biological considerations and impacts
L Wang - Accounts of chemical research, 2017 - ACS Publications
Conspectus Expansion of the genetic code allows unnatural amino acids (Uaas) to be site-
specifically incorporated into proteins in live biological systems, thus enabling novel …
specifically incorporated into proteins in live biological systems, thus enabling novel …
Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology
The genetic code, initially thought to be universal and immutable, is now known to contain
many variations, including biased codon usage, codon reassignment, ambiguous decoding …
many variations, including biased codon usage, codon reassignment, ambiguous decoding …
Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase
Pyrrolysyl-tRNA synthetase (PylRS) is a major tool in genetic code expansion using
noncanonical amino acids, yet its structure and function are not completely understood …
noncanonical amino acids, yet its structure and function are not completely understood …
Polyspecific pyrrolysyl-tRNA synthetases from directed evolution
Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNAPyl have emerged as ideal
translation components for genetic code innovation. Variants of the enzyme facilitate the …
translation components for genetic code innovation. Variants of the enzyme facilitate the …
Ancestral archaea expanded the genetic code with pyrrolysine
The pyrrolysyl-tRNA synthetase (PylRS) facilitates the cotranslational installation of the 22nd
amino acid pyrrolysine. Owing to its tolerance for diverse amino acid substrates, and its …
amino acid pyrrolysine. Owing to its tolerance for diverse amino acid substrates, and its …
Intra-and inter-nucleosomal interactions of the histone H4 tail revealed with a human nucleosome core particle with genetically-incorporated H4 tetra-acetylation
M Wakamori, Y Fujii, N Suka, M Shirouzu… - Scientific reports, 2015 - nature.com
Post-translational modifications (PTMs) of histones, such as lysine acetylation of the N-
terminal tails, play crucial roles in controlling gene expression. Due to the difficulty in …
terminal tails, play crucial roles in controlling gene expression. Due to the difficulty in …
Structural basis for genetic-code expansion with bulky lysine derivatives by an engineered pyrrolysyl-tRNA synthetase
T Yanagisawa, M Kuratani, E Seki, N Hino… - Cell Chemical …, 2019 - cell.com
Pyrrolysyl-tRNA synthetase (PylRS) and tRNA Pyl have been extensively used for genetic-
code expansion. A Methanosarcina mazei PylRS mutant bearing the Y306A and Y384F …
code expansion. A Methanosarcina mazei PylRS mutant bearing the Y306A and Y384F …
Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase
E Seki, T Yanagisawa, M Kuratani… - ACS Synthetic …, 2020 - ACS Publications
Pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs from Methanosarcina mazei and
Methanosarcina barkeri are widely used for site-specific incorporations of non-canonical …
Methanosarcina barkeri are widely used for site-specific incorporations of non-canonical …