Methodology of fish eDNA and its applications in ecology and environment

S Wang, Z Yan, B Hänfling, X Zheng, P Wang… - Science of the Total …, 2021 - Elsevier
Fish environmental DNA (eDNA) studies have made substantial progress during the past
decade, and significant advances in monitoring fishes have been gained by taking …

Scaling up: A guide to high‐throughput genomic approaches for biodiversity analysis

TM Porter, M Hajibabaei - Molecular ecology, 2018 - Wiley Online Library
The purpose of this review is to present the most common and emerging DNA‐based
methods used to generate data for biodiversity and biomonitoring studies. As environmental …

[图书][B] Environmental DNA: For biodiversity research and monitoring

P Taberlet, A Bonin, L Zinger, E Coissac - 2018 - books.google.com
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …

[HTML][HTML] Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding

H Krehenwinkel, M Wolf, JY Lim, AJ Rominger… - Scientific reports, 2017 - nature.com
Amplicon based metabarcoding promises rapid and cost-efficient analyses of species
composition. However, it is disputed whether abundance estimates can be derived from …

Why we need sustainable networks bridging countries, disciplines, cultures and generations for aquatic biomonitoring 2.0: a perspective derived from the DNAqua-Net …

F Leese, A Bouchez, K Abarenkov, F Altermatt… - Advances in ecological …, 2018 - Elsevier
Aquatic biomonitoring has become an essential task in Europe and many other regions as a
consequence of strong anthropogenic pressures affecting the health of lakes, rivers, oceans …

[HTML][HTML] Automated high throughput animal CO1 metabarcode classification

TM Porter, M Hajibabaei - Scientific Reports, 2018 - nature.com
We introduce a method for assigning names to CO1 metabarcode sequences with
confidence scores in a rapid, high-throughput manner. We compiled nearly 1 million CO1 …

Comparing diversity levels in environmental samples: DNA sequence capture and metabarcoding approaches using 18S and COI genes

H Giebner, K Langen, SJ Bourlat… - Molecular Ecology …, 2020 - Wiley Online Library
Environmental DNA studies targeting multiple taxa using metabarcoding provide remarkable
insights into levels of species diversity in any habitat. The main drawbacks are the presence …

[HTML][HTML] Putting COI metabarcoding in context: The utility of exact sequence variants (ESVs) in biodiversity analysis

TM Porter, M Hajibabaei - Frontiers in Ecology and Evolution, 2020 - frontiersin.org
DNA barcoding and metabarcoding are techniques that focus on signature genomic regions
that in theory provide species level resolution, but in practice this is not always possible. We …

[HTML][HTML] High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider …

SR Kennedy, S Prost, I Overcast, AJ Rominger… - … Genes and Evolution, 2020 - Springer
Large-scale studies on community ecology are highly desirable but often difficult to
accomplish due to the considerable investment of time, labor and, money required to …

Terrestrial mammal surveillance using hybridization capture of environmental DNA from African waterholes

PA Seeber, GK McEwen, U Löber… - Molecular ecology …, 2019 - Wiley Online Library
Determining species distributions can be extremely challenging but is crucial to ecological
and conservation research. Environmental DNA (eDNA) approaches have shown particular …