Deep 3D histology powered by tissue clearing, omics and AI

A Ertürk - Nature Methods, 2024 - nature.com
To comprehensively understand tissue and organism physiology and pathophysiology, it is
essential to create complete three-dimensional (3D) cellular maps. These maps require …

Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy

H Dibaji, A Kazemi Nasaban Shotorban… - Nature …, 2024 - nature.com
Rapid, high-resolution volumetric imaging without moving heavy objectives or disturbing
delicate samples remains challenging. Pupil-matched remote focusing offers a promising …

Mesoscopic oblique plane microscopy via light-sheet mirroring

S Daetwyler, BJ Chang, B Chen, FF Voigt, D Rajendran… - Optica, 2023 - opg.optica.org
Understanding the intricate interplay and inter-connectivity of biological processes across an
entire organism is important in various fields of biology, including cardiovascular research …

High‐Throughput 3D Imaging Flow Cytometry of Suspended Adherent 3D Cell Cultures

M Yamashita, M Tamamitsu, H Kirisako, Y Goda… - Small …, 2024 - Wiley Online Library
Abstract 3D cell cultures are indispensable in recapitulating in vivo environments. Among
the many 3D culture methods, culturing adherent cells on hydrogel beads to form spheroid …

Highly inclined light sheet allows volumetric super-resolution imaging of efflux pumps distribution in bacterial biofilms

T Vignolini, M Capitanio, C Caldini, L Gardini… - Scientific Reports, 2024 - nature.com
Bacterial biofilms are highly complex communities in which isogenic bacteria display
different gene expression patterns and organize in a three-dimensional mesh gaining …

Grating-free autofocus for single-pixel microscopic imaging

G Wang, H Deng, Y Cai, M Ma, X Zhong… - Photonics Research, 2024 - opg.optica.org
As a computational technology, single-pixel microscopic imaging (SPMI) transfers the
target's spatial information into a temporal dimension. The traditional focusing method of …

User-friendly oblique plane microscopy on a fully functional commercially available microscope base

G Sirinakis, ES Allgeyer, D Nashchekin… - Biomedical Optics …, 2024 - opg.optica.org
In this work we present an oblique plane microscope designed to work seamlessly with a
commercially available microscope base. To support all the functionality offered by the …

[PDF][PDF] 单物镜光片三维荧光成像技术研究进展(特邀)

张一凡, 李辉, 杨光 - Laser & Optoelectronics Progress, 2024 - researching.cn
摘要光片显微镜由于具有强大的光学层切能力, 较快的成像速度和较低的光损伤,
成为三维成像的重要工具. 光片显微镜通常利用两个垂直放置的物镜分别进行照明和成像 …

Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing

P Pozzi, V Balan, A Candeo, A Brix… - Journal of …, 2024 - spiedigitallibrary.org
Significance The reprojection setup typical of oblique plane microscopy (OPM) limits the
effective aperture of the imaging system, and therefore its efficiency and resolution. Large …

Metamaterials and plasmonics in Asia, a tribute to Byoungho Lee

QH Park, L Zhou, T Ishihara, JW Wu - Nanophotonics, 2023 - degruyter.com
This special issue is a development of the 6th A3 Metamaterials Forum held from June 27 to
29, 2022, at Seoul National University, Korea. Namkyoo Park, from Seoul National …