Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

P Intasian, K Prakinee, A Phintha, D Trisrivirat… - Chemical …, 2021 - ACS Publications
Since the industrial revolution, the rapid growth and development of global industries have
depended largely upon the utilization of coal-derived chemicals, and more recently, the …

Recent advances in simultaneous thermostability-activity improvement of industrial enzymes through structure modification

NG Nezhad, RNZR Abd Rahman, YM Normi… - International Journal of …, 2023 - Elsevier
Engineered thermostable microbial enzymes are widely employed to catalyze chemical
reactions in numerous industrial sectors. Although high thermostability is a prerequisite of …

A thermostable, closed SARS-CoV-2 spike protein trimer

X Xiong, K Qu, KA Ciazynska, M Hosmillo… - Nature structural & …, 2020 - nature.com
The spike (S) protein of SARS-CoV-2 mediates receptor binding and cell entry and is the
dominant target of the immune system. It exhibits substantial conformational flexibility. It …

Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation

M McCallum, AC Walls, JE Bowen, D Corti… - Nature structural & …, 2020 - nature.com
SARS-CoV-2 is the causative agent of the COVID-19 pandemic, with 10 million infections
and more than 500,000 fatalities by June 2020. To initiate infection, the SARS-CoV-2 spike …

Designing a novel multi-epitope vaccine to evoke a robust immune response against pathogenic multidrug-resistant Enterococcus faecium bacterium

J Dey, SR Mahapatra, TK Raj, T Kaur, P Jain, A Tiwari… - Gut Pathogens, 2022 - Springer
Enterococcus faecium is an emerging ESKAPE bacterium that is capable of causing severe
public health complications in humans. There are currently no licensed treatments or …

Exploring Klebsiella pneumoniae capsule polysaccharide proteins to design multiepitope subunit vaccine to fight against pneumonia

J Dey, SR Mahapatra, S Lata, S Patro… - Expert Review of …, 2022 - Taylor & Francis
Background Klebsiella pneumoniae is an emerging human pathogen causing neonatal lung
disease, catheter-associated infections, and nosocomial outbreaks with high fatality rates …

Exploring Leishmania secretory proteins to design B and T cell multi-epitope subunit vaccine using immunoinformatics approach

N Khatoon, RK Pandey, VK Prajapati - Scientific reports, 2017 - nature.com
Visceral leishmaniasis (VL) is a fatal form of leishmaniasis which affects 70 countries,
worldwide. Increasing drug resistance, HIV co-infection, and poor health system require …

Trimeric HIV-1-Env structures define glycan shields from clades A, B, and G

GBE Stewart-Jones, C Soto, T Lemmin, GY Chuang… - Cell, 2016 - cell.com
Summary The HIV-1-envelope (Env) trimer is covered by a glycan shield of∼ 90 N-linked
oligosaccharides, which comprises roughly half its mass and is a key component of HIV …

Advancing enzyme's stability and catalytic efficiency through synergy of force-field calculations, evolutionary analysis, and machine learning

A Kunka, SM Marques, M Havlasek, M Vasina… - ACS …, 2023 - ACS Publications
Thermostability is an essential requirement for the use of enzymes in the bioindustry. Here,
we compare different protein stabilization strategies using a challenging target, a stable …

[PDF][PDF] Protein stability: a crystallographer's perspective

MC Deller, L Kong, B Rupp - Acta Crystallographica Section F …, 2016 - journals.iucr.org
Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food
industries, in addition to being a daily consideration for academic researchers studying …