Errors occurring during DNA replication can result in inaccurate replication, incomplete
replication, or re-replication, resulting in genome instability that can lead to diseases such as …

The four-dimensional nucleome (4DN) consortium studies the architecture of the genome
and the nucleus in space and time. We summarize progress by the consortium and highlight …

Identifying the relationships between chromosome structures, nuclear bodies, chromatin
states and gene expression is an overarching goal of nuclear-organization studies …

The 3D organization of mammalian chromatin was described more than 30 years ago by
visualizing sites of DNA synthesis at different times during the S phase of the cell cycle …

Progress in development of biophysical analytic approaches has recently crossed paths with
macromolecule condensates in cells. These cell condensates, typically termed liquid-like …

The temporal order of DNA replication [replication timing (RT)] is correlated with chromatin
modifications and three-dimensional genome architecture; however, causal links have not …

Background DNA replication in mammalian cells occurs in a defined temporal order during
S phase, known as the replication timing (RT) programme. Replication timing is …

Active and inactive chromatin are spatially separated in the nucleus. In Hi-C data, this is
reflected by the formation of compartments, whose interactions form a characteristic …

Chromosome-wide late replication is an enigmatic hallmark of the inactive X chromosome
(Xi). How it is established and what it represents remains obscure. By single-cell DNA …

Cycling cells duplicate their DNA content during S phase, following a defined program
called replication timing (RT). Early-and late-replicating regions differ in terms of mutation …