The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Abstract The International Molecular Exchange (IMEx) Consortium provides scientists with a
single body of experimentally verified protein interactions curated in rich contextual detail to …

Transcription factors activate or repress target gene expression or switch between activation
and repression. In animals and yeast, Groucho/Tup1 corepressor proteins are recruited by …

Intracellular bacterial pathogens inject effector proteins to hijack host cellular processes and
promote their survival and proliferation. To systematically map effector-host protein-protein …

Background Yeast two-hybrid (Y2H) screens have been among the most powerful methods
to detect and analyze protein-protein interactions. However, they suffer from a significant …

Distinct cellular functions are executed by separate groups of proteins, organized into
complexes or functional modules, which are ultimately interconnected in cell-wide protein …

A major challenge in systems biology is to understand the gene regulatory networks that
drive development, physiology and pathology. Interactions between transcription factors and …

To the Editor: In a recent paper, Braun et al. 1 compared the ability of five different methods
to detect well-known protein-protein interactions (PPIs). The remarkable outcome of this …

Background Bacteriophage lambda is a model phage for most other dsDNA phages and has
been studied for over 60 years. Although it is probably the best-characterized phage there …

Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has
become an important technique for detecting physical interactions between sequence …