Comparison of two 16S rRNA primers (V3–V4 and V4–V5) for studies of arctic microbial communities

E Fadeev, MG Cardozo-Mino, JZ Rapp… - Frontiers in …, 2021 - frontiersin.org
Microbial communities of the Arctic Ocean are poorly characterized in comparison to other
aquatic environments as to their horizontal, vertical, and temporal turnover. Yet, recent …

Study of microbiome changes in patients with ulcerative colitis in the Central European part of Russia

MV Gryaznova, SA Solodskikh, AV Panevina… - Heliyon, 2021 - cell.com
Ulcerative colitis (UC) is an inflammatory disease that affects the colon and rectum.
Recently, evidence has emerged about the influence of microbiota on the development of …

Evaluation of primer pairs for microbiome profiling from soils to humans within the One Health framework

Wasimuddin, K Schlaeppi, F Ronchi… - Molecular ecology …, 2020 - Wiley Online Library
Abstract The 'One Health'framework emphasizes the ecological relationships between soil,
plant, animal and human health. Microbiomes play important roles in these relationships, as …

Metagenomics study in aquatic resource management: recent trends, applied methodologies and future needs

BK Behera, B Dehury, AK Rout, B Patra, N Mantri… - Gene Reports, 2021 - Elsevier
Due to continuous anthropogenic activities and other natural changes, aquatic ecosystems
are under increasing stress. Metagenomics aided by next-generation sequencing …

Seminal microbiota of idiopathic infertile patients and its relationship with sperm DNA integrity

S Garcia-Segura, J Del Rey, L Closa… - Frontiers in cell and …, 2022 - frontiersin.org
The development of new biomarkers for human male infertility is crucial to improve the
diagnosis and the prognosis of this disease. Recently, seminal microbiota was shown to be …

Diversity of bacterial community in the oxygen minimum zones of Arabian Sea and Bay of Bengal as deduced by illumina sequencing

GL Fernandes, BD Shenoy, SR Damare - Frontiers in microbiology, 2020 - frontiersin.org
The Indian Ocean harbors oxygen minimum zones (OMZs) in the Arabian Sea and Bay of
Bengal, with dissolved oxygen< 20 μM, located at the mid-depths of the water column. Till …

Simultaneous analysis of seven 16S rRNA hypervariable gene regions increases efficiency in marine bacterial diversity detection

K Leontidou, IL Abad‐Recio, V Rubel… - Environmental …, 2023 - Wiley Online Library
Environmental DNA sequencing is the gold standard to reveal microbial community
structures. In most applications, a one‐fragment PCR approach is applied to amplify a …

Supplementation of a lacto-fermented rapeseed-seaweed blend promotes gut microbial-and gut immune-modulation in weaner piglets

Y Hui, P Tamez-Hidalgo, T Cieplak, GD Satessa… - Journal of Animal …, 2021 - Springer
Background The direct use of medical zinc oxide in feed will be abandoned after 2022 in
Europe, leaving an urgent need for substitutes to prevent post-weaning disorders. Results …

Comparative microbiota characterization unveiled a contrasting pattern of floc-associated versus free-living bacterial communities in biofloc aquaculture

M Rajeev, I Jung, J Song, I Kang, JC Cho - Aquaculture, 2023 - Elsevier
Bacterial communities thriving in various components determine the host health and water
quality in biofloc technology (BFT)-based aquaculture system. Despite increasing utilization …

Fine-scale evaluation of two standard 16S rRNA gene amplicon primer pairs for analysis of total prokaryotes and archaeal nitrifiers in differently managed soils

J Zhao, J Rodriguez, W Martens-Habbena - Frontiers in Microbiology, 2023 - frontiersin.org
The advance of high-throughput molecular biology tools allows in-depth profiling of
microbial communities in soils, which possess a high diversity of prokaryotic …