Metabolite measurement: pitfalls to avoid and practices to follow
Metabolites are the small biological molecules involved in energy conversion and
biosynthesis. Studying metabolism is inherently challenging due to metabolites' reactivity …
biosynthesis. Studying metabolism is inherently challenging due to metabolites' reactivity …
Metabolomics for laboratory diagnostics
R Bujak, W Struck-Lewicka, MJ Markuszewski… - … of pharmaceutical and …, 2015 - Elsevier
Metabolomics is an emerging approach in a systems biology field. Due to continuous
development in advanced analytical techniques and in bioinformatics, metabolomics has …
development in advanced analytical techniques and in bioinformatics, metabolomics has …
The strengths and weaknesses of NMR spectroscopy and mass spectrometry with particular focus on metabolomics research
AHM Emwas - Metabonomics: Methods and protocols, 2015 - Springer
Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have evolved as the most
common techniques in metabolomics studies, and each brings its own advantages and …
common techniques in metabolomics studies, and each brings its own advantages and …
Understanding and manipulating the separation in hydrophilic interaction liquid chromatography
DV McCalley - Journal of chromatography A, 2017 - Elsevier
Hydrophilic interaction liquid chromatography (HILIC) has emerged as a valuable
complimentary technique to reversed-phase (RP), being especially suited for the analysis of …
complimentary technique to reversed-phase (RP), being especially suited for the analysis of …
Stationary and mobile phases in hydrophilic interaction chromatography: a review
P Jandera - Analytica chimica acta, 2011 - Elsevier
Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase
liquid chromatography separations of polar, weakly acidic or basic samples. In principle, this …
liquid chromatography separations of polar, weakly acidic or basic samples. In principle, this …
Current practice of liquid chromatography–mass spectrometry in metabolomics and metabonomics
Based on publication and citation numbers liquid chromatography (LC–MS) has become the
major analytical technology in the field of global metabolite profiling. This dominance reflects …
major analytical technology in the field of global metabolite profiling. This dominance reflects …
Liquid chromatography–mass spectrometry based global metabolite profiling: a review
Untargeted, global metabolite profiling (often described as metabonomics or metabolomics)
represents an expanding research topic and is, potentially, a major pillar for systems biology …
represents an expanding research topic and is, potentially, a major pillar for systems biology …
New advances in separation science for metabolomics: resolving chemical diversity in a post-genomic era
NL Kuehnbaum, P Britz-McKibbin - Chemical reviews, 2013 - ACS Publications
Metabolomics offers a revolutionary framework for phenotyping individuals at a molecular
level that is needed for new breakthroughs in cell biology and personalized medicine. 1− 3 …
level that is needed for new breakthroughs in cell biology and personalized medicine. 1− 3 …
Untargeted LC/MS-based metabolic phenotyping (metabonomics/metabolomics): The state of the art
Liquid chromatography (LC) hyphenated to mass spectrometry is currently the most widely
used means of determining metabolic phenotypes via both untargeted and targeted …
used means of determining metabolic phenotypes via both untargeted and targeted …
Toward 'omic scale metabolite profiling: a dual separation–mass spectrometry approach for coverage of lipid and central carbon metabolism
Although the objective of any 'omic science is broad measurement of its constituents, such
coverage has been challenging in metabolomics because the metabolome is comprised of a …
coverage has been challenging in metabolomics because the metabolome is comprised of a …