The yeast deletion collection: a decade of functional genomics

G Giaever, C Nislow - Genetics, 2014 - academic.oup.com
The yeast deletion collections comprise> 21,000 mutant strains that carry precise start-to-
stop deletions of∼ 6000 open reading frames. This collection includes heterozygous and …

Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries

KK Hong, J Nielsen - Cellular and Molecular Life Sciences, 2012 - Springer
Metabolic engineering is the enabling science of development of efficient cell factories for
the production of fuels, chemicals, pharmaceuticals, and food ingredients through microbial …

Rewiring carbon metabolism in yeast for high level production of aromatic chemicals

Q Liu, T Yu, X Li, Y Chen, K Campbell, J Nielsen… - Nature …, 2019 - nature.com
The production of bioactive plant compounds using microbial hosts is considered a safe,
cost-competitive and scalable approach to their production. However, microbial production …

Systems biology of metabolism

J Nielsen - Annual review of biochemistry, 2017 - annualreviews.org
Metabolism is highly complex and involves thousands of different connected reactions; it is
therefore necessary to use mathematical models for holistic studies. The use of …

Engineering plant secondary metabolism in microbial systems

ME Pyne, L Narcross, VJJ Martin - Plant physiology, 2019 - academic.oup.com
Engineering Plant Secondary Metabolism in Microbial Systems | Plant Physiology | Oxford
Academic Skip to Main Content Advertisement Oxford Academic Journals Books Search Menu …

A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case …

I Nookaew, M Papini, N Pornputtapong… - Nucleic acids …, 2012 - academic.oup.com
RNA-seq, has recently become an attractive method of choice in the studies of
transcriptomes, promising several advantages compared with microarrays. In this study, we …

Real-time metabolome profiling of the metabolic switch between starvation and growth

H Link, T Fuhrer, L Gerosa, N Zamboni, U Sauer - Nature methods, 2015 - nature.com
Metabolic systems are often the first networks to respond to environmental changes, and the
ability to monitor metabolite dynamics is key for understanding these cellular responses …

Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry

AD Silverman, N Kelley-Loughnane… - ACS synthetic …, 2018 - ACS Publications
Recent advances in cell-free gene expression (CFE) systems have enabled their use for a
host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and …

[HTML][HTML] CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains

V Stovicek, I Borodina, J Forster - Metabolic Engineering Communications, 2015 - Elsevier
There is a demand to develop 3rd generation biorefineries that integrate energy production
with the production of higher value chemicals from renewable feedstocks. Here, robust and …

De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial …

JF Nijkamp, M van den Broek, E Datema, S de Kok… - Microbial cell …, 2012 - Springer
Abstract Saccharomyces cerevisiae CEN. PK 113-7D is widely used for metabolic
engineering and systems biology research in industry and academia. We sequenced …