Precursor messenger RNA (pre-mRNA) splicing is a critical step in the posttranscriptional
regulation of gene expression, providing significant expansion of the functional proteome of …

The pathway from gene activation in the nucleus to mRNA translation and decay at specific
locations in the cytoplasm is both streamlined and highly interconnected. This review …

Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and
introns are removed from pre-mRNA by the spliceosome. Understanding the time lag …

New roles for RNAs in biology continue to emerge, and a glance at the history of RNAs may
prepare molecular biologists for future discoveries about these powerful molecules. A …

There is little quantitative information regarding how much splicing occurs co-
transcriptionally in higher eukaryotes, and it remains unclear where precisely splicing occurs …

To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced
nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. Eighty …

Self-cleaving ribozymes were discovered 30 years ago, but their biological distribution and
catalytic mechanisms are only beginning to be defined. Each ribozyme family is defined by a …

Mounting evidence suggests that transcription and RNA processing are intimately coupled
in vivo, although each process can occur independently in vitro. It is generally thought that …

Many steps of RNA processing occur during transcription by RNA polymerases. Co-
transcriptional activities are deemed commonplace in prokaryotes, in which the lack of …

Transcription and splicing are functionally coupled, resulting in highly efficient splicing of
RNA polymerase II (RNAP II) transcripts. The mechanism involved in this coupling is not …