Imaging biofilms using fluorescence in situ hybridization: seeing is believing

A Barbosa, S Miranda, NF Azevedo… - Frontiers in Cellular …, 2023 - frontiersin.org
Biofilms are complex structures with an intricate relationship between the resident
microorganisms, the extracellular matrix, and the surrounding environment. Interest in …

[HTML][HTML] The transcriptional regulators of virulence for Pseudomonas aeruginosa: Therapeutic opportunity and preventive potential of its clinical infections

X Shao, C Yao, Y Ding, H Hu, G Qian, M He, X Deng - Genes & Diseases, 2023 - Elsevier
In Pseudomonas aeruginosa (P. aeruginosa), transcription factors (TFs) are important
mediators in the genetic regulation of adaptability and pathogenicity to respond to multiple …

Machine-learning from Pseudomonas putida KT2440 transcriptomes reveals its transcriptional regulatory network

HG Lim, K Rychel, AV Sastry, GJ Bentley, J Mueller… - Metabolic …, 2022 - Elsevier
Bacterial gene expression is orchestrated by numerous transcription factors (TFs).
Elucidating how gene expression is regulated is fundamental to understanding bacterial …

Advanced transcriptomic analysis reveals the role of efflux pumps and media composition in antibiotic responses of Pseudomonas aeruginosa

A Rajput, H Tsunemoto, AV Sastry… - Nucleic acids …, 2022 - academic.oup.com
Pseudomonas aeruginosa is an opportunistic pathogen and major cause of hospital-
acquired infections. The virulence of P. aeruginosa is largely determined by its …

Systems biology of competency in Vibrio natriegens is revealed by applying novel data analytics to the transcriptome

J Shin, K Rychel, BO Palsson - Cell Reports, 2023 - cell.com
Vibrio natriegens regulates natural competence through the TfoX and QstR transcription
factors, which are involved in external DNA capture and transport. However, the extensive …

A multi-scale expression and regulation knowledge base for Escherichia coli

CR Lamoureux, KT Decker, AV Sastry… - Nucleic Acids …, 2023 - academic.oup.com
Transcriptomic data is accumulating rapidly; thus, scalable methods for extracting
knowledge from this data are critical. Here, we assembled a top-down expression and …

Laboratory evolution, transcriptomics, and modeling reveal mechanisms of paraquat tolerance

K Rychel, J Tan, A Patel, C Lamoureux, Y Hefner… - Cell reports, 2023 - cell.com
Relationships between the genome, transcriptome, and metabolome underlie all evolved
phenotypes. However, it has proved difficult to elucidate these relationships because of the …

A comprehensive review of genomics, transcriptomics, proteomics, and metabolomic insights into the differentiation of Pseudomonas aeruginosa from the planktonic …

M Vohra, A Kour, NP Kalia, M Kumar, S Sharma… - International Journal of …, 2023 - Elsevier
Biofilm formation by Pseudomonas aeruginosa is primarily responsible for chronic wound
and lung infections in humans. These infections are persistent owing to the biofilm's high …

Compendium-wide analysis of Pseudomonas aeruginosa core and accessory genes reveals transcriptional patterns across strains PAO1 and PA14

AJ Lee, G Doing, SL Neff, T Reiter, DA Hogan… - …, 2023 - Am Soc Microbiol
Pseudomonas aeruginosa is an opportunistic pathogen that causes difficult-to-treat
infections. Two well-studied divergent P. aeruginosa strain types, PAO1 and PA14, have …

The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions

C Dalldorf, K Rychel, R Szubin, Y Hefner, A Patel… - Msystems, 2024 - Am Soc Microbiol
Fast growth phenotypes are achieved through optimal transcriptomic allocation, in which
cells must balance tradeoffs in resource allocation between diverse functions. One such …