In humans, the thyroid hormones T3 and T4 are synthesized in the thyroid gland in a
process that crucially involves the iodoglycoprotein thyroglobulin. The overall structure of …

Mass spectrometry is well-established as one of the primary methods used to characterize
peptides and proteins. New advances in instrumentation and innovative analytical workflows …

Ion mobility brings an additional dimension of separation to LC–MS, improving identification
of peptides and proteins in complex mixtures. A recently introduced timsTOF mass …

Protein glycosylation, an important PTM, plays an essential role in a wide range of biological
processes such as immune response, intercellular signaling, inflammation, and host …

Protein glycosylation is one of the most important posttranslational modifications. Numerous
biological functions are related to protein glycosylation. However, analytical challenges …

We report the comparison of mass-spectral-based abundances of tryptic glycopeptides to
fluorescence abundances of released labeled glycans and the effects of mass and charge …

Sialic acids are a family of structurally unique and negatively charged nine‐carbon sugars,
normally found at the terminal positions of glycan chains on glycoproteins and glycolipids …

Abstract Analysis of glycosylation is challenging due to micro‐and macro‐heterogeneity of
the protein attachment. A combination of LC with MS/MS is one of the most powerful tools for …

Retention time is one of the most important parameters that has been widely used to
demonstrate the separation results obtained from liquid chromatography (LC) platforms …

Protein-based therapeutics such as mAbs have become emerging drugs in modern
medicine. Most of the approved therapeutic proteins are glycoproteins. Glycosylation is an …