Characterization of lipid–protein interactions and lipid-mediated modulation of membrane protein function through molecular simulation
The cellular membrane constitutes one of the most fundamental compartments of a living
cell, where key processes such as selective transport of material and exchange of …
cell, where key processes such as selective transport of material and exchange of …
Three-dimensional electron crystallography of protein microcrystals
We demonstrate that it is feasible to determine high-resolution protein structures by electron
crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM) …
crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM) …
MicroED: conception, practice and future opportunities
This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021.
The cryo-EM method microcrystal electron diffraction is described and put in the context of …
The cryo-EM method microcrystal electron diffraction is described and put in the context of …
Ab initio phasing macromolecular structures using electron-counted MicroED data
Structures of two globular proteins were determined ab initio using microcrystal electron
diffraction (MicroED) data that were collected on a direct electron detector in counting mode …
diffraction (MicroED) data that were collected on a direct electron detector in counting mode …
[PDF][PDF] MicroED data collection and processing
MicroED, a method at the intersection of X-ray crystallography and electron cryo-microscopy,
has rapidly progressed by exploiting advances in both fields and has already been …
has rapidly progressed by exploiting advances in both fields and has already been …
Use of transmission electron microscopy to identify nanocrystals of challenging protein targets
HP Stevenson, AM Makhov, M Calero… - Proceedings of the …, 2014 - National Acad Sciences
The current practice for identifying crystal hits for X-ray crystallography relies on optical
microscopy techniques that are limited to detecting crystals no smaller than 5 μm. Because …
microscopy techniques that are limited to detecting crystals no smaller than 5 μm. Because …
Electron diffraction and three-dimensional crystallography for structural biology
MTB Clabbers, JP Abrahams - Crystallography Reviews, 2018 - Taylor & Francis
Electron crystallography is increasingly becoming a viable alternative for structure
elucidation of three-dimensional, multi-nanometre sized crystals of beam-sensitive organics …
elucidation of three-dimensional, multi-nanometre sized crystals of beam-sensitive organics …
MicroED opens a new era for biological structure determination
BL Nannenga, T Gonen - Current opinion in structural biology, 2016 - Elsevier
Highlights•MicroED is a recently developed method for CryoEM.•MicroED can be used to
solve structures from crystals much smaller than those used for X-ray crystallography.• …
solve structures from crystals much smaller than those used for X-ray crystallography.• …
The influence of lipids on voltage-gated ion channels
Voltage-gated ion channels are responsible for transmitting electrochemical signals in both
excitable and non-excitable cells. Structural studies of voltage-gated potassium and sodium …
excitable and non-excitable cells. Structural studies of voltage-gated potassium and sodium …
Experimental phasing of MicroED data using radiation damage
We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to
determine atomic-resolution structures from vanishingly small three-dimensional crystals …
determine atomic-resolution structures from vanishingly small three-dimensional crystals …