Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Navigation through the bulk tumour, entry into the blood vasculature, survival in the
circulation, exit at distant sites and resumption of proliferation are all steps necessary for …

The function of a T cell depends on its subtype and activation state. Here, we show that
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …

Metastasis is the spread of cancer cells from a primary tumor to distant sites within the body
to establish secondary tumors. Although this is an inefficient process, the consequences are …

Significance: Optical imaging using the endogenous fluorescence of metabolic cofactors has
enabled nondestructive examination of dynamic changes in cell and tissue function both in …

The possibility of detecting and classifying living cells in a label-free and non-invasive
manner holds significant theranostic potential. In this work, Hyperspectral Imaging (HSI) has …

More than 60 years ago, the idea was introduced that NADH autofluorescence could be
used as a marker of cellular redox state and indirectly also of cellular energy metabolism …

The function of macrophages in vitro is linked to their metabolic rewiring. However,
macrophage metabolism remains poorly characterized in situ. Here, we used two-photon …

Neural computations occurring simultaneously in multiple cerebral cortical regions are
critical for mediating behaviors. Progress has been made in understanding how neural …

ABSTRACT A major focus of current biological studies is to fill the knowledge gaps between
cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical …