The development of new ion-activation/dissociation methods continues to be one of the most
active areas of mass spectrometry owing to the broad applications of tandem mass …

Beyond the genetic code, the human proteome is greatly diversified by genetic variations,
alternative splicing of RNA transcripts, and post-translational modifications (PTMs). 6, 7 In …

The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …

Top-down proteomics by mass spectrometry (MS) involves the mass measurement of an
intact protein followed by subsequent activation of the protein to generate product ions …

Recent advances in top-down mass spectrometry strategies continue to improve the
analysis of intact proteins. 193 nm ultraviolet photodissociation (UVPD) is one method well …

Ultraviolet photodissociation (UVPD) produces rich and informative fragmentation of intact
protein ions, but in the case of high mass proteins (> 30 kDa) the spectra are congested with …

Understanding and elucidating the diverse structures and functions of lipids has motivated
the development of many innovative tandem mass spectrometry (MS/MS) strategies. Higher …

Polyubiquitination is an important post-translational modification (PTM) that regulates
various biological functions. The linkage sites and topologies of polyubiquitination chains …

Direct sampling of lipids from tissues for direct mass spectrometry (MS) analysis allows a
quick profiling of lipidome, which is important for biomedical applications. In this work, we …

Ultraviolet photodissociation (UVPD) mass spectrometry has gained attention in recent
years for its ability to provide high sequence coverage of intact proteins. However …